Effect of lengthening lymphocyte function-associated antigen 3 on adhesion to CD2.
Effect of lengthening lymphocyte function-associated antigen 3 on adhesion to CD2.
复制标题
延长淋巴细胞功能相关抗原 3 对 CD2 粘附的影响。
DOI：
10.1091/mbc.3.2.157
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发表时间：
1992
影响因子：
3.3
通讯作者：
Springer,TA
中科院分区：
文献类型：
--
作者：
Chan,PY;Springer,TA
通过将四个Ig样结构域插入两个IG结构域淋巴细胞功能相关抗原3（LFA-3）分子中，研究了延长受体结合位点和膜锚之间的粘附分子距离的效果。在中国仓鼠卵巢（CHO）细胞中表达的延伸分子在4 ℃下与T淋巴细胞上的CD 2结合的效率比野生型分子高4至20倍。用神经氨酸酶处理CHO克隆以去除唾液酸，或用脱氧甘露尻霉素处理以减少大量N-连接的糖基化，表明与野生型和嵌合LFA-3分子的粘附在细胞-细胞排斥力的影响下达到相似的程度，并且这些处理的效果小于延长LFA-3。在更高的温度下，例如22和37 ° C，与野生型LFA-3的结合效率增加到与延伸的LFA-3的结合相当的水平。我们的研究结果表明，从细胞膜锚的粘合剂结合位点的更远的位置，通过提高受体遇到配体的频率增加细胞-细胞粘附的效率，并且粘合剂结合位点的更近的位置可以在生理温度下提供有效的细胞-细胞粘附。
The effect of lengthening the distance in an adhesion molecule between the receptor binding site and the membrane anchor was studied by inserting four Ig-like domains into the two Ig domain lymphocyte function-associated antigen 3 (LFA-3) molecule. The extended molecule expressed in Chinese hamster ovary (CHO) cells bound to CD2 on T lymphocytes 4- to 20-fold more efficiently than the wild-type molecule at 4 degrees C. Treatment of the CHO clones with neuraminidase to remove sialic acid, or with deoxymannojirimycin to reduce the bulk of N-linked glycosylation, showed that adhesion to both the wild-type and the chimeric LFA-3 molecules was under the influence of cell-cell repulsive forces to a similar extent and that these treatments had less effect than lengthening LFA-3. At higher temperatures, such as 22 and 37 degrees C, the efficiency of binding to the wild-type LFA-3 increased to levels comparable with binding to extended LFA-3. Our results suggest that more distal locations of the adhesive binding site from the cell membrane anchor increase the efficiency of cell-cell adhesion by enhancing the frequency of receptor encounter with ligand and that more proximal locations of the adhesive binding site can provide efficient cell-cell adhesion at physiological temperatures.
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影响因子：
4.4
作者：
S. Denning;Debbi T. Tuck;L. Vollger;T. Springer;K. Singer;B. Haynes
通讯作者：
S. Denning;Debbi T. Tuck;L. Vollger;T. Springer;K. Singer;B. Haynes
影响因子：
4.4
作者：
N. Hollander;P. Selvaraj;T. Springer
通讯作者：
N. Hollander;P. Selvaraj;T. Springer
人绒毛膜促性腺激素-α链的膜锚定形式而非分泌形式获得聚乳糖胺聚糖。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fukuda,M;Guan,JL;Rose,JK
通讯作者：
Rose,JK
影响因子：
64.8
作者：
FUHRMANN, U;BAUSE, E;PLOEGH, H
通讯作者：
PLOEGH, H
影响因子：
64.8
作者：
PETERSON, A;SEED, B
通讯作者：
SEED, B