The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production
The effect of serum-free media on the metabolic yields and growth rates of C2C12 cells in the context of cultivated meat production
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无血清培养基对培养肉生产中 C2C12 细胞代谢产量和生长速率的影响
DOI：
10.1016/j.fufo.2023.100226
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Block, David E.
中科院分区：
文献类型：
--
作者：
O'Neill, Edward N.;Neffling, Milla;Randall, Nick;Kwong, Grace;Ansel, Joshua;Baar, Keith;Block, David E.
成功的商业化种植的肉类产品作为替代来源的膳食蛋白质为日益增长的全球人口将需要开发高效和廉价的无血清细胞培养基。众所周知，无血清培养基与基于血清的培养基相比引起不同的细胞生长行为，但全面探索无血清培养物对培养的肉类相关细胞类型的营养需求的影响的数据尚未报道。我们对在Essential 8无血清培养基和常规含血清培养基中生长的C2 C12细胞进行了用过的培养基分析。数据表明，虽然在两种培养基中7天的细胞生长速率相似，但在一些关键营养物质如葡萄糖、谷氨酰胺、甘氨酸和胱氨酸的利用率方面存在显着差异。我们从数据中推断，生产1 kg C2 C12细胞需要大约250-275 g氨基酸和1100-1500 g葡萄糖，尽管当使用更优化的细胞、培养基和补料策略时，这些要求可能会改变。我们的研究结果强调了血清和血清替代品对细胞代谢的影响，并表明以前使用含血清培养基进行的代谢研究的数据可能无法直接转化为无血清系统。
Successful commercialization of cultivated meat products as alternative sources of dietary protein for a growing global population will require development of efficient and inexpensive serum-free cell culture media. It is known that serum-free media elicit dissimilar cell growth behavior compared to serum-based media, but data comprehensively exploring the effects of serum-free culture on the nutrient requirements of cultivated meat-relevant cell types have yet to be reported. We performed spent media analysis of C2C12 cells growing in Essential 8 serum-free media and in conventional serum-containing media. Data indicated that although the cell growth rates were similar in the two media over seven days, there were significant differences in the utilization rates of some key nutrients such as glucose, glutamine, glycine, and cystine. We extrapolated from the data that roughly 250–275 g of amino acids and 1100–1500 g of glucose would be required to produce 1 kg of C2C12 cells, though these requirements may change when using more optimized cells, media, and feed strategies. Our results highlight the influence of serum and serum replacements on cell metabolism, and indicate that previous data from metabolic studies performed using serum-containing media may not directly translate to serum-free systems.
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影响因子：
6.4
作者：
O'Neill, Edward N.;Ansel, Joshua C.;Kwong, Grace A.;Plastino, Michael E.;Nelson, Jenny;Baar, Keith;Block, David E.
通讯作者：
Block, David E.
影响因子：
4.7
作者：
Cosenza, Zachary;Block, David E.;Baar, Keith
通讯作者：
Baar, Keith
影响因子：
4.6
作者：
Jang M;Scheffold J;Røst LM;Cheon H;Bruheim P
通讯作者：
Bruheim P
影响因子：
48
作者：
Chen, Guokai;Gulbranson, Daniel R.;Hou, Zhonggang;Bolin, Jennifer M.;Ruotti, Victor;Probasco, Mitchell D.;Smuga-Otto, Kimberly;Howden, Sara E.;Diol, Nicole R.;Propson, Nicholas E.;Wagner, Ryan;Lee, Garrett O.;Antosiewicz-Bourget, Jessica;Teng, Joyce M. C.;Thomson, James A.
通讯作者：
Thomson, James A.
改进的化学计量模型在生物反应器动物细胞培养基设计和补料分批培养中的应用
DOI：
--
发表时间：
2004
期刊：
Cytotechnology (Dordrecht)
影响因子：
--
作者：
Liangzhi Xie;Daniel I. C. Wang
通讯作者：
Daniel I. C. Wang