Spent media analysis suggests cultivated meat media will require species and cell type optimization.
Spent media analysis suggests cultivated meat media will require species and cell type optimization.
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DOI：
10.1038/s41538-022-00157-z
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发表时间：
2022-09-29
影响因子：
6.4
通讯作者：
Block, David E.
中科院分区：
文献类型：
--
作者：
O'Neill, Edward N.;Ansel, Joshua C.;Kwong, Grace A.;Plastino, Michael E.;Nelson, Jenny;Baar, Keith;Block, David E.
细胞培养基的设计可能是目前面临的最重要的障碍，商业化的养殖肉类作为替代来源的膳食蛋白质。由于特定培养系统的培养基优化需要大量的努力和投资，因此仍然存在的一个主要问题是培养基配方是否可以在不同物种和谱系的细胞的多个生产方案中轻松共享。在这里，我们进行用过的培养基分析，比较特定的营养利用的原代鸡胚肌肉前体细胞和成纤维细胞的小鼠C2C12成肌细胞系。我们证明，这些相关的细胞类型有显着不同的营养利用模式集体和每个细胞的基础上，和传统媒体的许多组件不出现被耗尽的细胞。也就是说，与其他细胞相比，鸡肌肉前体细胞消耗葡萄糖的速度不快，也不完全，并且在培养的第一天，其他几种关键营养素的特定消耗率存在显着差异。最终，我们的研究结果表明，没有一种培养基可能是理想的和具有成本效益的培养多种细胞类型，并且简化培养基优化工作的新方法对行业发展至关重要。
Cell culture media design is perhaps the most significant hurdle currently facing the commercialization of cultivated meat as an alternative source of dietary protein. Since media optimization for a specific culture system requires a significant amount of effort and investment, a major question remaining is whether media formulations can be easily shared across multiple production schemes for cells of different species and lineages. Here, we perform spent medium analysis to compare the specific nutrient utilization of primary embryonic chicken muscle precursor cells and fibroblasts to the murine C2C12 myoblast cell line. We demonstrate that these related cell types have significantly different nutrient utilization patterns collectively and on a per-cell basis, and that many components of conventional media do not appear to be depleted by the cells. Namely, glucose was not consumed as rapidly nor as completely by the chicken muscle precursors compared to other cells overall, and there were significant differences in specific consumption rates for several other key nutrients over the first day of culture. Ultimately, our results indicate that no one medium is likely ideal and cost effective to culture multiple cell types and that novel methods to streamline media optimization efforts will be important for the industry to develop.
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影响因子：
12.4
作者：
Li, Yong;Li, Juan;Huard, Johnny
通讯作者：
Huard, Johnny
影响因子：
5.5
作者：
Hu, Lin-Ya;Mileti, Cassidy J.;Smith, Lucas R.
通讯作者：
Smith, Lucas R.
影响因子：
2.9
作者：
Okamoto, Yuta;Haraguchi, Yuji;Shimizu, Tatsuya
通讯作者：
Shimizu, Tatsuya
影响因子：
1.5
作者：
Bereiter-Hahn, J;Münnich, A;Woiteneck, P
通讯作者：
Woiteneck, P
DOI：
10.1016/j.bbrc.2014.06.123
发表时间：
2014-07-25
影响因子：
3.1
作者：
Kanzleiter, Timo;Rath, Michaela;Eckardt, Kristin
通讯作者：
Eckardt, Kristin