Regulation of growth hormone binding protein in man: comparison of gel chromatography and immunoprecipitation methods.
Regulation of growth hormone binding protein in man: comparison of gel chromatography and immunoprecipitation methods.
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人体生长激素结合蛋白的调节：凝胶色谱法和免疫沉淀法的比较。
DOI：
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发表时间：
1993
影响因子：
5.8
通讯作者：
I. Rajkovic
中科院分区：
文献类型：
--
作者：
K. Ho;E. Valiontis;M. Waters;I. Rajkovic
生长激素部分与高亲和力结合蛋白（GHBP）结合循环。凝胶色谱法是测定血浆中生长激素结合活性的常用方法，但速度慢且繁琐。利用生长激素受体单克隆抗体免疫沉淀分离结合生长激素和游离生长激素可能是一个更实际的选择。我们研究了生长激素和雌激素状态对24小时池样本中GHBP的影响，并比较了凝胶过滤和免疫沉淀得到的结果。GHBP活性（[125I]GH特异性结合百分比）在正常、GH缺乏和肢端肥大症受试者中测量；两组绝经后妇女分别在口服（乙炔雌二醇20微克/天）或透皮（17 -雌二醇100微克/天）雌激素治疗前后。GHBP活性在正常、GHBP缺乏和肢端肥大症受试者之间的年龄和性别没有显著差异。无论是GH缺乏和正常受试者，还是奥曲肽治疗肢端肥大症患者，GHBP活性都没有明显改变。口服雌激素治疗显著增加GHBP活性。Scatchard分析显示，这种变化与结合能力的增加有关（P = 0.001），但与亲和力无关。经皮雌激素治疗后GHBP活性无明显变化。两种方法获得的GHBP活性显著相关（n = 70, r = 0.92, P = 0.001），尽管抗体法的值高25%。同样，容量估计值高度相关（r = 0.90, P = 0.0001），免疫沉淀的值高出40%。我们的结论是生长激素分泌状态不是GHBP的决定因素。口服雌激素通过增加GHBP的容量而不是亲和力来增加GHBP活性。雌激素的作用是途径依赖的，口服反应可能反映了第一次肝脏作用。免疫沉淀估计的更高的结合活性和容量可能反映了更快速的分离过程，从而最大限度地减少了GHBP复合物的解离。免疫沉淀法测定血清中GH结合活性和GHBP浓度具有简单、方便、准确等优点。
GH circulates in part bound to a high affinity binding protein (GHBP). Gel chromatography is the established method for measuring GH binding activity in plasma, but is slow and tedious. The separation of bound from free GH by immunoprecipitation using a monoclonal antibody to the GH receptor may be a more practical alternative. We have examined the effects of GH and estrogen status on GHBP measured in 24-h pool samples and compared results obtained from gel filtration and immunoprecipitation. GHBP activity (percent specific binding of [125I]GH) was measured in normal, GH-deficient, and acromegalic subjects; and in two groups of postmenopausal women before and after oral (ethinyl estradiol 20 micrograms daily) or transdermal (17 beta-estradiol 100 micrograms daily) estrogen therapy. GHBP activity was not significantly different between normal, GH-deficient, and acromegalic subjects matched for age and sex. Neither GH administration to GH-deficient and normal subjects, nor octreotide treatment of patients with acromegaly, significantly altered GHBP activity. Oral estrogen treatment significantly increased GHBP activity. This change was associated with an increase in binding capacity (P = 0.001) but not affinity, as revealed by Scatchard analysis. GHBP activity did not change significantly with transdermal estrogen therapy. GHBP activity obtained by the two methods was significantly correlated (n = 70, r = 0.92, P = 0.001) although values for the antibody method were 25% higher. Similarly, capacity estimates were highly correlated (r = 0.90, P = 0.0001), with values by immunoprecipitation being 40% higher. We conclude that GH secretory status is not a determinant of GHBP. Oral estrogens increase GHBP activity through an increase in capacity and not affinity. The estrogen effect is route dependent and the oral response is likely to reflect a first pass hepatic effect. The higher binding activity and capacity estimated from immunoprecipitation are likely to reflect a more rapid separation process thereby minimizing dissociation of GHBP complex. Immunoprecipitation offers the advantages of simplicity, convenience, and accuracy for the measurement of GH binding activity and GHBP concentrations in serum.
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人血浆中第二种亲和力较低的生长激素结合蛋白。
DOI：
10.1210/jcem-70-3-680
发表时间：
1990
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Baumann,G;Shaw,MA
通讯作者：
Shaw,MA
影响因子：
4.8
作者：
MAITER, D;UNDERWOOD, LE;KETELSLEGERS, JM
通讯作者：
KETELSLEGERS, JM
生长激素受体缺乏症（拉伦侏儒症）患者血清生长激素结合蛋白缺失。
DOI：
10.1073/pnas.84.13.4636
发表时间：
1987
影响因子：
11.1
作者：
Daughaday,WH;Trivedi,B
通讯作者：
Trivedi,B
循环生长激素结合蛋白对人类生长激素代谢清除、分布和降解的影响。
DOI：
10.1210/jcem-64-4-657
发表时间：
1987
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Baumann,G;Amburn,KD;Buchanan,TA
通讯作者：
Buchanan,TA
健康和疾病中血浆生长激素结合蛋白的调节。
DOI：
10.1016/0026-0495(89)90108-x
发表时间：
1989
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
Baumann,G;Shaw,MA;Amburn,K
通讯作者：
Amburn,K