Genome-wide analyses of genes encoding FK506-binding proteins reveal their involvement in abiotic stress responses in apple.
Genome-wide analyses of genes encoding FK506-binding proteins reveal their involvement in abiotic stress responses in apple.
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对编码 FK506 结合蛋白的基因进行全基因组分析揭示了它们参与苹果非生物胁迫反应
DOI：
10.1186/s12864-018-5097-8
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发表时间：
2018-09-25
期刊：
影响因子：
4.4
通讯作者：
Ma F
中科院分区：
文献类型：
--
作者：
Dong Q;Mao K;Duan D;Zhao S;Wang Y;Wang Q;Huang D;Li C;Liu C;Gong X;Ma F
FK506结合蛋白(FKBP)在植物生长、发育和非生物胁迫反应的许多关键过程中发挥着不同的作用。然而，FKBP基因家族在重要的果树作物苹果(Malus×home tica Borkh.)没有像在其他物种中那样被彻底地研究。我们的研究目的是探讨苹果FKBPs使苹果植物耐受非生物胁迫的机制。利用基于生物信息学的方法、RT-PCR和qRT-PCR技术，我们鉴定了38个FKBP基因，并在苹果基因组中克隆了其中16个基因。系统发育分析揭示了该家族中的三个主要群体。序列比对、三维结构、系统发育和保守结构域分析的结果表明，苹果FKBPs在结构和结构上高度保守。此外，基因组结构分析表明，这些基因在其他几个物种中也高度保守。综合QRT-PCR分析发现，MdFKBPs在不同组织和植物对水分和盐胁迫的反应中表达模式不同。根据互作网络和共表达分析的结果，我们确定了MdFKBP62a/MdFKBP65a/b介导的网络中的配对参与了水分胁迫和盐胁迫信号的传递，这两种信号都通过与苹果热激蛋白的相互作用而统一上调。这些结果为进一步研究FKBP基因及其在苹果非生物胁迫反应和多种代谢生理过程中的功能提供了新的思路。本文的在线版本(10.1186/s12864-0185097-8)包含向授权用户提供的补充材料。
The FK506-binding proteins (FKBPs) play diverse roles in numerous critical processes for plant growth, development, and abiotic stress responses. However, the FKBP gene family in the important fruit crop apple (Malus × domestica Borkh.) has not been studied as thoroughly as in other species. Our research objective was to investigate the mechanisms by which apple FKBPs enable apple plants to tolerate the effects of abiotic stresses. Using bioinformatics-based methods, RT-PCR, and qRT-PCR technologies, we identified 38 FKBP genes and cloned 16 of them in the apple genome. The phylogenetic analysis revealed three major groups within that family. The results from sequence alignments, 3-D structures, phylogenetics, and analyses of conserved domains indicated that apple FKBPs are highly and structurally conserved. Furthermore, genomics structure analysis showed that those genes are also highly and structurally conserved in several other species. Comprehensive qRT-PCR analysis found various expression patterns for MdFKBPs in different tissues and in plant responses to water-deficit and salt stresses. Based on the results from interaction network and co-expression analyses, we determined that the pairing in the MdFKBP62a/MdFKBP65a/b-mediated network is involved in water-deficit and salt-stress signaling, both of which are uniformly up-regulated through interactions with heat shock proteins in apple. These results provide new insight for further study of FKBP genes and their functions in abiotic stress response and multiple metabolic and physiological processes in apple. The online version of this article (10.1186/s12864-018-5097-8) contains supplementary material, which is available to authorized users.
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影响因子：
5.3
作者：
Ahn JC;Kim DW;You YN;Seok MS;Park JM;Hwang H;Kim BG;Luan S;Park HS;Cho HS
通讯作者：
Cho HS
影响因子：
5.1
作者：
Aviezer-Hagai, Keren;Skovorodnikova, Julia;Breiman, Adina
通讯作者：
Breiman, Adina
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影响因子：
4.6
作者：
Hu W;Wang L;Tie W;Yan Y;Ding Z;Liu J;Li M;Peng M;Xu B;Jin Z
通讯作者：
Jin Z
影响因子：
3.7
作者：
Cao Z;Zhang G;Wang F;Liu H;Liu L;Han Y;Zhang J;Yuan J
通讯作者：
Yuan J
影响因子：
4.8
作者：
Bouchard, Rodolphe;Bailly, Aurelien;Geisler, Markus
通讯作者：
Geisler, Markus