TM4SF1 promotes the self-renewal of esophageal cancer stem-like cells and is regulated by miR-141.
TM4SF1 promotes the self-renewal of esophageal cancer stem-like cells and is regulated by miR-141.
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TM4SF1促进食管癌干细胞样细胞自我更新并受miR-141调控
DOI：
10.18632/oncotarget.13866
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发表时间：
2017-03-21
期刊：
影响因子：
--
通讯作者：
Lu SH
中科院分区：
文献类型：
--
作者：
Xue L;Yu X;Jiang X;Deng X;Mao L;Guo L;Fan J;Fan Q;Wang L;Lu SH
已经在原发性人类肿瘤和癌细胞系中鉴定出癌症干细胞样细胞。以前我们发现TM 4SF 1基因在食管鳞状细胞癌（esophageal squamous cell carcinoma，ESCC）细胞系的SP细胞中高表达，但其在ESCC中的作用及其机制尚不清楚。在本研究中，我们观察到TM 4SF 1在从ESCC细胞系分离的SP细胞中上调，而miR-141在SP细胞中下调。TM 4SF 1可刺激食管癌干细胞样细胞的自我更新能力和致癌性，促进细胞的侵袭和迁移。在miR-141过表达的细胞中，TM 4SF 1的表达显著降低。我们还发现过表达miR-141可以通过抑制TM 4SF 1来消除食管癌干细胞样细胞的自我更新能力和致癌性，并减少细胞的侵袭和迁移。因此，TM 4SF 1是miR-141的直接靶基因。miR-141对TM 4SF 1的调控可能在调控食管癌干细胞样细胞自我更新中发挥重要作用。它还可能促进新的治疗策略和有效药物的开发，以靶向ESCC干细胞样细胞。
Cancer stem-like cells have been identified in primary human tumors and cancer cell lines. Previously we found TM4SF1 gene was highly expressed in side population (SP) cells from esophageal squamous cell carcinoma (ESCC) cell lines, but the role and underlying mechanism of TM4SF1 in ESCC remain unclear. In this study, we observed TM4SF1 was up-regulated but miR-141 was down-regulated in SP cells isolated from ESCC cell lines. TM4SF1 could stimulate the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells, and promote cell invasion and migration. In miR-141 overexpression cells, the expression of TM4SF1 was significantly reduced. We also found that overexpression of miR-141 could abolish the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells and decrease cell invasion and migration by suppressing TM4SF1. Consequently, TM4SF1 is a direct target gene of miR-141. The regulation of TM4SF1 by miR-141 may play an important role in controlling self-renewals of esophageal cancer stem-like cells. It may also promote the development of new therapeutic strategies and efficient drugs to target ESCC stem-like cells.
影响因子：
2.9
作者：
Shi HY;Zhu SC;Shen WB;Liu ML
通讯作者：
Liu ML