Rapid rise of the ESBL and mcr-1 genes in Escherichia coli of chicken origin in China, 2008-2014.
Rapid rise of the ESBL and mcr-1 genes in Escherichia coli of chicken origin in China, 2008-2014.
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2008-2014年中国鸡源性大肠杆菌ESBL和mcr-1基因快速上升
DOI：
10.1038/s41426-018-0033-1
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发表时间：
2018-03-14
影响因子：
13.2
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Wu C;Wang Y;Shi X;Wang S;Ren H;Shen Z;Wang Y;Lin J;Wang S
产生广谱β-内酰胺酶的大肠杆菌（esblc - ec）菌株正在世界各地出现，成为对氨苄西林、头孢噻肟和头孢他啶等β-内酰胺类抗生素耐药的来源。McR-1是一种新的质粒介导的耐粘菌素基因。本研究的目的是调查2008-2014年中国不同省份鸡源性ESBL-EC mcr-1的流行情况。总体而言，821株分离株中有341株为ESBL-EC株，2008 - 2014年esbl阳性株的比例几乎翻了一番。研究结果显示了地区差异，山东集中的家禽业区的ESBL-EC分离株明显多于其他四省。ESBL型分析显示，blaCTX-M基因是最常见的ESBL编码基因（92.7%）。共检测到CTX-M基因12个亚型，其中鉴定出的主要基因型为blaCTX-M-55（34.3%）和blaCTX-M-65（17.9%）。此外，86.0%的菌株携带blaTEM和8.5%的菌株携带pAmpC基因。在本研究中，我们还观察到44株不同ST型（ST46、ST1286、ST10、ST29、ST101和ST354）携带mcr-1的大肠杆菌，其中大部分携带mcr-1的质粒为IncI2。全基因组测序分析表明，blaCTX-M和mcr-1在动物和人类来源的ESBL- ec中共存，系统发育分析进一步揭示了它们之间的密切关系，特别是有几个分离株具有少量的snp，这表明ESBL和mcr-1在临床医学和兽医学中共存和传播的趋势正在增加。
Extended-spectrum beta-lactamase-producing Escherichia coli (ESBL-EC) strains are emerging around the world as a source of resistance to β-lactam antibiotics such as ampicillin, cefotaxime, and ceftazidime. mcr-1 is a novel plasmid-mediated gene conferring resistance to colistin. The aim of this study was to investigate the prevalence of ESBL-EC mcr-1 of chicken origin in the different provinces of China during 2008–2014. Overall, 341 of 821 isolates were determined to be ESBL-EC strains, and the proportion of ESBL-positive strains almost doubled from 2008 to 2014. The findings of our study revealed regional differences, with significantly more ESBL-EC isolates from stockbreeding in concentrated poultry industry areas in Shandong than from the other four provinces. The ESBL type analysis showed that blaCTX-M was the most prevalent ESBL-encoding gene (92.7%). In total, twelve subtypes of CTX-M genes were detected, among which, blaCTX-M-55 (34.3%) and blaCTX-M-65 (17.9%) were the major identified genotypes. In addition, blaTEM and pAmpC genes were carried by 86.0% and 8.5% of isolates, respectively. In this study, we also observed 44 E. coli isolates with multiple ST types (ST46, ST1286, ST10, ST29, ST101, and ST354) carrying mcr-1, and the majority of mcr-1–carrying plasmids were IncI2. The whole-genome sequencing analysis indicated the co-existence of blaCTX-M and mcr-1 in ESBL-EC of both animal and human origin, and phylogenetic analysis further revealed their close relationship, especially several isolates sharing a small number of SNPs, which suggested the increasing trend of co-existence and transmission of ESBL and mcr-1 in both clinical medicine and veterinary medicine.
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