Survey of Ixodes pacificus Ticks in California Reveals a Diversity of Microorganisms and a Novel and Widespread Anaplasmataceae Species.
Survey of Ixodes pacificus Ticks in California Reveals a Diversity of Microorganisms and a Novel and Widespread Anaplasmataceae Species.
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DOI：
10.1371/journal.pone.0135828
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Ecker DJ
中科院分区：
文献类型：
--
作者：
Eshoo MW;Carolan HE;Massire C;Chou DM;Crowder CD;Rounds MA;Phillipson CA;Schutzer SE;Ecker DJ
太平洋硬蜱可携带多种人类和动物病原体。为了调查蜱传的已知和假定病原体的流行情况，我们测试了982个个体的成虫和若虫I。2007年至2009年期间在加州各地收集的太平洋蜱，使用宽范围PCR和电喷雾电离质谱（PCR/ESI-MS）检测法，旨在检测各种蜱传微生物。总体而言，发现1.4%的蜱感染伯氏疏螺旋体，2.0%感染宫本疏螺旋体，0.3%感染嗜吞噬细胞无形体。此外，3.0%的人感染了巴氏线虫。约1.2%的蜱同时感染一种以上的病原体或假定病原体。此外，我们确定了一个新的无形体科物种，我们的特点是其16 S rRNA，groEL，gltA，和rpoB基因的测序。序列分析表明，这种生物体在遗传上与已知的无形体属物种不同，其最近的遗传近邻来自亚洲。这种新型无形体科物种的流行率在一个地点高达21%，在全州范围内检测到4.9%的蜱虫。基于这种遗传特征，我们建议将这种生物体称为“加州隐原体”。对这种新型微生物的了解将为社区提供对I. pacificus微生物组，这种细菌可以更广泛传播的概念;以及探索这种细菌是否也有助于人类或动物疾病负担的机会。
Ixodes pacificus ticks can harbor a wide range of human and animal pathogens. To survey the prevalence of tick-borne known and putative pathogens, we tested 982 individual adult and nymphal I. pacificus ticks collected throughout California between 2007 and 2009 using a broad-range PCR and electrospray ionization mass spectrometry (PCR/ESI-MS) assay designed to detect a wide range of tick-borne microorganisms. Overall, 1.4% of the ticks were found to be infected with Borrelia burgdorferi, 2.0% were infected with Borrelia miyamotoi and 0.3% were infected with Anaplasma phagocytophilum. In addition, 3.0% were infected with Babesia odocoilei. About 1.2% of the ticks were co-infected with more than one pathogen or putative pathogen. In addition, we identified a novel Anaplasmataceae species that we characterized by sequencing of its 16S rRNA, groEL, gltA, and rpoB genes. Sequence analysis indicated that this organism is phylogenetically distinct from known Anaplasma species with its closest genetic near neighbors coming from Asia. The prevalence of this novel Anaplasmataceae species was as high as 21% at one site, and it was detected in 4.9% of ticks tested statewide. Based upon this genetic characterization we propose that this organism be called ‘Candidatus Cryptoplasma californiense’. Knowledge of this novel microbe will provide awareness for the community about the breadth of the I. pacificus microbiome, the concept that this bacterium could be more widely spread; and an opportunity to explore whether this bacterium also contributes to human or animal disease burden.
影响因子：
13.2
作者：
Rounds, Megan A.;Crowder, Christopher D.;Stratton, Charles W.;Ecker, David J.;Eshoo, Mark W.;Tang, Yi-Wei
通讯作者：
Tang, Yi-Wei