Detection of Plasmodium vivax in a child returning from India by use of broad-range PCR and electrospray ionization mass spectrometry.

Detection of Plasmodium vivax in a child returning from India by use of broad-range PCR and electrospray ionization mass spectrometry.
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DOI:
10.1038/emi.2012.49
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发表时间:
2012-12
影响因子:
13.2
通讯作者:
Tang, Yi-Wei
Tang, Yi-Wei
中科院分区:
医学2区
文献类型:
--
作者:
Rounds, Megan A.;Crowder, Christopher D.;Stratton, Charles W.;Ecker, David J.;Eshoo, Mark W.;Tang, Yi-Wei

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间日疟原虫是美国输入性疟疾的常见原因,仅次于恶性疟原虫。我们介绍了一名从印度返回的儿童感染间日疟原虫的病例。间日疟原虫最初通过标准方法进行诊断,并通过广范围 PCR 和电喷雾电离质谱法使用一组设计用于检测媒介传播病原体的引物进行回顾性检测。这是首次报道使用 PCR 和电喷雾电离质谱法检测间日疟原虫的病例。
Plasmodium vivax is a common cause of imported malaria in the USA, second only to P. falciparum. We present a case of P. vivax malaria in a child returning from India. P. vivax was initially diagnosed by standard methodology and detected retrospectively by use of broad-range PCR and electrospray ionization mass spectrometry using a panel of primers designed to detect vector-borne pathogens. This is the first reported case of P. vivax detection using PCR and electrospray ionization mass spectrometry.
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