Functional validation of novel levamisole resistance marker S168T in Haemonchus contortus
Functional validation of novel levamisole resistance marker S168T in Haemonchus contortus
复制标题
捻转血矛线虫新型左旋咪唑抗性标记S168T的功能验证
DOI：
10.1016/j.ijpddr.2024.100524
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Antonopoulos A
中科院分区：
文献类型：
--
作者：
Antonopoulos A
Recently, a S168T variant in the acetylcholine receptor subunit ACR-8 was associated with levamisole resistance in the parasitic helminthHaemonchus contortus. Here, we used theXenopus laevisoocyte expression system and two-electrode voltage-clamp electrophysiology to measure the functional impact of this S168T variant on theH. contortuslevamisole-sensitive acetylcholine receptor, L-AChR-1.1. Expression of the ACR-8 S168T variant significantly reduced the current amplitude elicited by levamisole compared to acetylcholine, with levamisole changing from a full to partial agonist on the recombinant L-AChR. Functional validation of the S168T mutation on modulating levamisole activity at the receptor level highlights its critical importance as both a mechanism and a marker of levamisole resistance.
影响因子：
4.5
作者：
Choi YJ;Bisset SA;Doyle SR;Hallsworth-Pepin K;Martin J;Grant WN;Mitreva M
通讯作者：
Mitreva M
影响因子：
7.3
作者：
J. Aceves;D. Erlij;R. Martinez
通讯作者：
R. Martinez