Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel.
Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel.
复制标题
在纤维蛋白凝胶上，用大鼠新生儿心脏细胞自发地收缩心脏组织斑块。
DOI：
10.1002/term.1895
复制
发表时间：
2017-01
影响因子：
3.3
通讯作者：
Birla, Ravi K.
中科院分区：
文献类型：
--
作者：
Tao, Ze-Wei;Mohamed, Mohamed;Hogan, Matthew;Gutierrez, Laura;Birla, Ravi K.
工程心脏组织是用原代或干细胞衍生的心脏细胞在天然或合成支架上构建的。通过增加张力支持和输送足够的细胞，它们代表了治疗受伤区域的巨大潜力。本研究将100万~ 600万新生心肌细胞接种于纤维蛋白凝胶上制备心肌组织贴片，测定培养时间和细胞密度对自发收缩率、抽动力和节律反应频率的影响。并分析心电图和连接蛋白43的信号体积指数。1-6M细胞密度的斑块在电镀后的前4天内收缩速率在305-410 bpm之间；低细胞密度（1-3M）斑块持续节律性收缩的时间长于高细胞密度（4-6M）。细胞密度为1-6 M的斑块在第4-6天产生的收缩力在2.245-14.065 mN/mm3之间。在斑块形成后，获得了约6 Hz的节奏响应频率，在培养6天后下降到约3 Hz。高细胞密度斑块含有较厚的真心脏组织层，产生较高的R波振幅；而低密度斑块的connexin 43信号体积指数较大。此外，所有斑块均显示内皮细胞生长和核分裂旺盛。目前的研究表明，这种心脏结构的体内植入时间正好是斑块形成的时候，3-4M细胞密度的斑块是最佳的选择。
Engineered cardiac tissues have been constructed with primary or stem cell-derived cardiac cells on natural or synthetic scaffolds. They represent a tremendous potential for treatment of injured areas through addition of tensional support and delivery of sufficient cells. In this study 1 to 6 million (M) neonatal cardiac cells were seeded on fibrin gels to fabricate cardiac tissue patches, and the effects of culture time and cell density on spontaneous contraction rates, twitch forces and paced response frequencies were measured. Electrocardiograms and signal volume index of connexin 43 were also analyzed. Patches of 1–6M cell densities exhibited maximal contraction rates between 305–410 bpm within the first 4 days after plating; low cell densities (1–3M) patches sustained rhythmic contraction longer than high cell densities (4–6M). Patches with 1–6 M cell densities generated contractile forces in the range 2.245–14.065 mN/mm3 on days 4–6. Upon patch formation, a paced response frequency of approximately 6 Hz was obtained, and decreased to approximately 3 Hz after 6 days of culture. High cell density patches contained a thicker real cardiac tissue layer which generated higher R wave amplitudes; however, low density patches had a greater signal volume index of connexin 43. In addition, all patches manifested endothelial cell growth and robust nuclear division. The present study demonstrates that the proper time for in vivo implantation of this cardiac construct is just at patch formation and patches with 3–4M cell densities are the best candidates.
登录
查看更多内容
影响因子：
3.7
作者：
Han YS;Ogut O
通讯作者：
Ogut O
影响因子：
20.1
作者：
Tulloch NL;Muskheli V;Razumova MV;Korte FS;Regnier M;Hauch KD;Pabon L;Reinecke H;Murry CE
通讯作者：
Murry CE
DOI：
10.1073/pnas.0908381106
发表时间：
2009-09-29
影响因子：
11.1
作者：
Stevens, K. R.;Kreutziger, K. L.;Murry, C. E.
通讯作者：
Murry, C. E.
影响因子：
3.7
作者：
MARK, GE;STRASSER, FF
通讯作者：
STRASSER, FF
影响因子：
7.3
作者：
Salameh, A.;Krautblatter, S.;Janousek, J.
通讯作者：
Janousek, J.