Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation.
Self-Prepared Hyaluronic Acid/Alkaline Gelatin Composite with Nano-Hydroxyapatite and Bone Morphogenetic Protein for Cranial Bone Formation.
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DOI：
10.3390/ijms24021104
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发表时间：
2023-01-06
影响因子：
5.6
通讯作者：
Kondo, Hisatomo
中科院分区：
文献类型：
--
作者：
Hachinohe, Yuki;Taira, Masayuki;Hoshi, Miki;Yoshida, Daichi;Hatakeyama, Wataru;Sawada, Tomofumi;Kondo, Hisatomo
关键词：
新型骨形成替代材料在牙科种植学中非常有用。本研究的目的是制备交联透明质酸（cHLA）/交联碱性明胶（cAG）/纳米羟基磷灰石（nHAp）/骨形态发生蛋白（BMP）复合材料，并评价其在大鼠颅骨缺损中的成骨能力。将用环氧交联剂处理的cHLA和cAG液体以3：1的体积比混合，然后冷冻干燥。将干燥的复合材料进一步用仅含nHAp的水（BMP（-））或用含nHAp和BMP的水（BMP（+））渗透。将制备的湿结构（BMP（-）和BMP（+））植入大鼠颅骨缺损中，同时也制作仅缺损，并喂养动物8周，随后进行软X射线测量和组织学观察。X线结果显示，BMP（+）结构消失，但导致周围骨从缺损边缘向内延伸，长度增加约24%，大于BMP（-）结构和缺损，分别增加约17%和8%（p < 0.05）。BMP（+）构建体样本的组织学观察清楚地表明了由岛状骨阵列组成的活性骨延伸。结论：cHLA/cAG/nHAp/BMP可作为新型骨替代材料。
New bone-forming substitute materials are highly useful in dental implantology. The purpose of this study was to prepare cross-linked hyaluronic acid (cHLA)/cross-linked alkaline gelatin (cAG)/nano-hydroxyapatite (nHAp)/bone morphogenic protein (BMP) constructs; and evaluate their bone-forming capabilities in rat cranial bone defects. The cHLA and cAG liquids processed with an epoxy cross-linker were blended with a 3:1 volume ratio, followed by freeze-drying. The dry composites were further infiltrated with water containing nHAp only (BMP (−)) or with water containing nHAp and BMP (BMP (+)). Prepared wet constructs (BMP (−) and BMP (+)) were implanted in rat cranial bone defects, while defects only were also made, and animals were fed for 8 weeks, followed by subsequent soft X-ray measurements and histological observations. The X-ray results showed that BMP (+) constructs disappeared, though caused inward extension of peripherical bone from defect edges with an increase in length of approximately 24%, larger than those of BMP (−) constructs and defect only with approximately 17% and 8% increments, respectively (p < 0.05). Histological observations of BMP (+) construct samples clearly indicated active bone extension consisting of an array of island-like bones. It was concluded that cHLA/cAG/nHAp/BMP could be used as novel bone-substitute materials.
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影响因子：
5.4
作者：
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通讯作者：
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影响因子：
6.2
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通讯作者：
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