Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea.
Heritable variation and small RNAs in the progeny of chimeras of Brassica juncea and Brassica oleracea.
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芥菜和甘蓝嵌合体后代的遗传变异和小RNA
DOI：
10.1093/jxb/ert266
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发表时间：
2013-11
影响因子：
6.9
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Li J;Wang Y;Zhang L;Liu B;Cao L;Qi Z;Chen L
嵌合体已被用于研究遗传物质的传递和由此产生的遗传变异。本研究以两个嵌合体TCC和TTC（茎尖分生组织的外、中、内细胞层的起源分别用“T”（榨菜）和“C”（红甘蓝）表示）及其无性和有性后代为材料，分析了嫁接诱导变异的机理和遗传。通过不定芽再生获得TCC无性后代，通过自交获得TTC有性后代。本研究观察到类似的形态变化，在无性和有性后代，包括叶片形状的变化和拍摄顶端分生组织终止的模式。TTC后代的叶片形态变异稳定，但顶端分生组织终止率在连续3轮自交后由74.52%下降到3.01%。在红甘蓝的无性再生植株（rTTT）中发现了特异的小RNA，而在TTT中没有，表明小RNA可能通过嫁接从红甘蓝传递到榨菜中。此外，与在后代中观察到的表型变化平行，一些保守的miRNA在rTTT和TTT中差异表达，这与其靶基因表达的变化相关。这些结果表明，嫁接诱导的小RNA表达变化可能是嫁接诱导遗传变异的一个重要因素，为进一步通过表观遗传学研究嫁接诱导遗传变异的机制提供了基础。
Chimeras have been used to study the transmission of genetic material and the resulting genetic variation. In this study, two chimeras, TCC and TTC (where the origin of the outer, middle, and inner cell layers, respectively, of the shoot apical meristem is designated by a ‘T’ for tuber mustard and ‘C’ for red cabbage), as well as their asexual and sexual progeny, were used to analyse the mechanism and the inheritance of the variation induced by grafting. Asexual TCC progeny were obtained by adventitious shoot regeneration, while TTC sexual progeny were produced by self-crossing. This study observed similar morphological variations in both the asexual and sexual progeny, including changes in leaf shape and the pattern of shoot apical meristem termination. The leaf shape variation was stable, while the rate of shoot apical meristem termination in the TTC progenies decreased from 74.52% to 3.01% after three successive rounds of self-crossing. Specific red cabbage small RNAs were found in the asexually regenerated plants (rTTT) that were not present in TTT, indicating that small RNAs might be transmitted from red cabbage to tuber mustard during grafting. Moreover, in parallel with the variations in phenotype observed in the progeny, some conserved miRNAs were differentially expressed in rTTT and TTT, which correlated with changes in expression of their target genes. These results suggest that the change in small RNA expression induced by grafting may be an important factor for introducing graft-induced genetic variations, providing a basis for further investigating the mechanism of graft-induced genetic variation through epigenetics.
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影响因子：
7.2
作者：
Pant, Bikram Datt;Buhtz, Anja;Kehr, Julia;Scheible, Wolf-Ruediger
通讯作者：
Scheible, Wolf-Ruediger
影响因子：
56.9
作者：
Jacobsen, SE;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
3.5
作者：
Haque, A. K. M. Namul;Yamaoka, Naoto;Nishiguchi, Masamichi
通讯作者：
Nishiguchi, Masamichi
影响因子：
1.9
作者：
OHTA, Y
通讯作者：
OHTA, Y
影响因子：
1.9
作者：
OHTA, Y;VANCHUONG, P
通讯作者：
VANCHUONG, P