Analysis of volatile organic compounds from deep airway in the lung through intubation sampling
Analysis of volatile organic compounds from deep airway in the lung through intubation sampling
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通过插管取样分析肺部深部气道的挥发性有机化合物
DOI：
10.1007/s00216-022-04295-x
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发表时间：
2022-08
影响因子：
4.3
通讯作者：
Yannan Chu
中科院分区：
文献类型：
--
作者：
Wei Xu;Jin Zhang;Houwen Ding;Yueting Ding;Xue Zou;Min Yang;Qiang Zhou;Zhou Liu;Ling Zheng;Heping Zuo;Dianlong Ge;Qiangling Zhang;Chaoqun Huang;Chengyin Shen;Yannan Chu
Exhaled volatile organic compounds (VOCs) have been widely applied for the study of disease biomarkers. Oral exhalation and nasal exhalation are two of the most common sampling methods. However, VOCs released from food residues and bacteria in the mouth or upper respiratory tract were also sampled and usually mistaken as that produced from body metabolism. In this study, exhalation from deep airway was first directly collected through intubation sampling and analyzed. The exhalation samples of 35 subjects were collected through a catheter, which was inserted into the trachea or bronchus through the mouth and upper respiratory tract. Then, the VOCs in these samples were detected by proton transfer reaction mass spectrometry (PTR-MS). In addition, fast gas chromatography proton transfer reaction mass spectrometry (FGC-PTR-MS) was used to further determine the VOCs with the same mass-to-charge ratios. The results showed that there was methanol, acetonitrile, ethanol, methyl mercaptan, acetone, isoprene, and phenol in the deep airway. Compared with that in oral exhalation, ethanol, methyl mercaptan, and phenol had lower concentrations. In detail, the median concentrations of ethanol, methyl mercaptan, and phenol were 7.3, 0.6, and 23.9 ppbv, while those in the oral exhalation were 80.0, 5.1, and 71.3 ppbv, respectively, which meant the three VOCs mainly originated from the food residues and bacteria in the mouth or upper respiratory tract, rather than body metabolism. The research results in our study can provide references for expiratory VOC research based on oral and nasal exhalation samplings, which are more feasible in clinical practice.Graphical abstract
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影响因子：
2.8
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通讯作者：
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4.8
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--
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3.7
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9.5
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影响因子：
9.3
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通讯作者：
Odom John AR