Identification of a multipotent self-renewing stromal progenitor population during mammalian kidney organogenesis.
Identification of a multipotent self-renewing stromal progenitor population during mammalian kidney organogenesis.
复制标题
DOI：
10.1016/j.stemcr.2014.08.008
复制
发表时间：
2014-10-14
影响因子：
5.9
通讯作者：
McMahon, Andrew P.
中科院分区：
文献类型：
--
作者：
Kobayashi, Akio;Mugford, Joshua W.;Krautzberger, A. Michaela;Naiman, Natalie;Liao, Jessica;McMahon, Andrew P.
哺乳动物的肾脏是一个由多种细胞类型组成的复杂器官。我们先前的研究表明，表达SIX2的CaP间充质细胞是肾脏基本功能单位肾单位主体的多能自我更新祖细胞群体。然而，建立间质组织的细胞机制还不太清楚。我们证明，表达Foxd1的皮质基质代表了一个独特的多潜能自我更新的祖细胞群体，它在整个肾脏器官发生过程中形成了间质、系膜和周细胞的间质组织。对表达Foxd1的细胞的命运图分析表明，在肾脏发育的早期阶段，这些细胞中的一小部分有助于表达SIX2的细胞。此后，分别由表达SIX2和表达Foxd1的细胞类型形成了严格的肾单位和基质谱系边界。综上所述，我们的观察表明，在哺乳动物肾脏器官发生过程中，不同的多潜能自我更新祖细胞群体协调肾单位上皮和肾基质的细胞分化。Foxd1+皮质基质是一个多能自我更新的基质祖细胞池在肾单位和基质祖细胞隔间形成谱系边界一小部分Foxd1+细胞在早期成为SIX2+肾单位祖细胞虽然器官功能单位(也称为“实质”)的祖细胞群体已有较好的研究，但周围基质细胞的起源在许多器官中不太清楚，包括肾脏。在这篇文章中，小林和他的同事们发现了哺乳动物肾脏发育过程中一个多潜能的自我更新的基质祖细胞群体。
The mammalian kidney is a complex organ consisting of multiple cell types. We previously showed that the Six2-expressing cap mesenchyme is a multipotent self-renewing progenitor population for the main body of the nephron, the basic functional unit of the kidney. However, the cellular mechanisms establishing stromal tissues are less clear. We demonstrate that the Foxd1-expressing cortical stroma represents a distinct multipotent self-renewing progenitor population that gives rise to stromal tissues of the interstitium, mesangium, and pericytes throughout kidney organogenesis. Fate map analysis of Foxd1-expressing cells demonstrates that a small subset of these cells contributes to Six2-expressing cells at the early stage of kidney outgrowth. Thereafter, there appears to be a strict nephron and stromal lineage boundary derived from Six2-expressing and Foxd1-expressing cell types, respectively. Taken together, our observations suggest that distinct multipotent self-renewing progenitor populations coordinate cellular differentiation of the nephron epithelium and renal stroma during mammalian kidney organogenesis. The Foxd1+ cortical stroma is a multipotent self-renewing stromal progenitor pool A lineage boundary forms between the nephron and stromal progenitor compartments A small fraction of Foxd1+ cells becomes Six2+ nephron progenitors at early stages Although progenitor populations for functional units of organs (also known as “parenchyma”) have been relatively well studied, origins of surrounding stromal cells are less clear in many organs, including the kidney. In this article, Kobayashi and colleagues discovered a multipotent self-renewing stromal progenitor population during mammalian kidney development.
登录
查看更多内容
影响因子：
11.8
作者：
Carroll, TJ;Park, JS;McMahon, AP
通讯作者：
McMahon, AP
GUDMAP数据库 - 用于泌尿生殖器研究的在线资源。
DOI：
10.1242/dev.063594
发表时间：
2011-07
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Harding SD;Armit C;Armstrong J;Brennan J;Cheng Y;Haggarty B;Houghton D;Lloyd-MacGilp S;Pi X;Roochun Y;Sharghi M;Tindal C;McMahon AP;Gottesman B;Little MH;Georgas K;Aronow BJ;Potter SS;Brunskill EW;Southard-Smith EM;Mendelsohn C;Baldock RA;Davies JA;Davidson D
通讯作者：
Davidson D
影响因子：
14.9
作者：
Indra, AK;Warot, X;Metzger, D
通讯作者：
Metzger, D
影响因子：
2.7
作者：
Boyle, Scott;Misfeldt, Andrew;de Caestecker, Mark
通讯作者：
de Caestecker, Mark
影响因子：
2.7
作者：
Bohnenpoll, Tobias;Bettenhausen, Eva;Kispert, Andreas
通讯作者：
Kispert, Andreas