Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model.
Proteomic analysis of six- and twelve-month hippocampus and cerebellum in a murine Down syndrome model.
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DOI：
10.1016/j.neurobiolaging.2017.11.010
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发表时间：
2018-03
影响因子：
4.2
通讯作者：
Patterson D
中科院分区：
文献类型：
--
作者：
Vacano GN;Gibson DS;Turjoman AA;Gawryluk JW;Geiger JD;Duncan M;Patterson D
本研究旨在研究唐氏综合征Ts65Dn小鼠模型的脑蛋白质组。我们分析了小脑和海马蛋白质组的6个月和12个月大的三体和二体小鼠的差异凝胶电泳。我们量化了2082个蛋白质点的水平，并通过质谱法鉴定了272个（170个独特的UniProt加入物）。在Ts65Dn小鼠中，四种鉴定的蛋白质由基因三体编码。其中三种（EZL11、EZR和SOD1）升高，p值< 0.05，两种由二体基因编码的蛋白质（MAPRE3和PHB）降低。基于年龄（6个月与12个月）和大脑区域（小脑与海马）的凝胶间比较显示出许多差异。具体来说，132个识别的蛋白质在年龄组之间是不同的，141个识别的蛋白质在两个大脑区域之间是不同的。我们的研究结果表明，补偿机制存在，改善了Ts65Dn小鼠的三体性的影响。在衰老过程中观察到的差异可能在Ts65Dn小鼠的学习和记忆加速恶化中发挥作用。
This study was designed to investigate the brain proteome of the Ts65Dn mouse model of Down syndrome. We profiled the cerebellum and hippocampus proteomes of six and twelve-month-old trisomic and disomic mice by Difference Gel Electrophoresis. We quantified levels of 2082 protein spots and identified 272 (170 unique UniProt accessions) by mass spectrometry. Four identified proteins are encoded by genes trisomic in the Ts65Dn mouse. Three of these (CRYZL11, EZR, and SOD1) were elevated with p value < 0.05, and two proteins encoded by disomic genes (MAPRE3 and PHB) were reduced. Inter-gel comparisons based on age (six versus twelve-month) and brain region (cerebellum versus hippocampus) revealed numerous differences. Specifically, 132 identified proteins were different between age groups, and 141 identified proteins were different between the two brain regions. Our results suggest that compensatory mechanisms exist which ameliorate the effect of trisomy in the Ts65Dn mice. Differences observed during aging may play a role in the accelerated deterioration of learning and memory seen in Ts65Dn mice.
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影响因子：
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作者：
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