Bacterial population succession and adaptation affected by insecticide application and soil spraying history.
Bacterial population succession and adaptation affected by insecticide application and soil spraying history.
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DOI：
10.3389/fmicb.2014.00457
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发表时间：
2014
影响因子：
5.2
通讯作者：
Kikuchi Y
中科院分区：
文献类型：
--
作者：
Itoh H;Navarro R;Takeshita K;Tago K;Hayatsu M;Hori T;Kikuchi Y
虽然微生物群落有不同程度的暴露于环境压力，如化学污染，很少有人知道这些社区如何应对环境干扰，以及过去的干扰历史如何影响这些社区水平的反应。为全面了解有机磷农药施用对土壤微生物区系的影响，采用培养试验和16 SrRNA基因深度测序的方法，研究了杀螟松[O，O-二甲基O-（3-甲基-对硝基苯基）硫代磷酸酯，简称MEP]对土壤微生物群落的影响。尽管在初始阶段微生物组成相似，微生物对MEP施用的反应在有和没有MEP喷洒历史的土壤之间存在显著差异。MEP降解菌在有MEP喷洒历史的土壤中增加更快，表明土壤中MEP降解菌可能已经存在一定水平，并能迅速响应MEP再处理。培养依赖性和非依赖性的评价表明，MEP降解伯克霍尔德氏菌在MEP应用后的土壤中占主导地位，其中有限的成员可能在土壤中的MEP降解中发挥关键作用。值得注意的是，深度测序还揭示了一些甲基营养菌在MEP应用后显著增加，强烈表明这些细菌在消耗和去除甲醇（MEP降解的有害衍生物）中发挥作用，以使MEP降解细菌更好地生长。这项综合性的研究表明，MEP应用下的微生物群落的演替和适应过程，这是过去的经验，农药喷洒的严重影响。
Although microbial communities have varying degrees of exposure to environmental stresses such as chemical pollution, little is known on how these communities respond to environmental disturbances and how past disturbance history affects these community-level responses. To comprehensively understand the effect of organophosphorus insecticide application on microbiota in soils with or without insecticide-spraying history, we investigated the microbial succession in response to the addition of fenitrothion [O,O-dimethyl O-(3-methyl-p-nitrophenyl) phosphorothioate, abbreviated as MEP] by culture-dependent experiments and deep sequencing of 16S rRNA genes. Despite similar microbial composition at the initial stage, microbial response to MEP application was remarkably different between soils with and without MEP-spraying history. MEP-degrading microbes more rapidly increased in the soils with MEP-spraying history, suggesting that MEP-degrading bacteria might already exist at a certain level and could quickly respond to MEP re-treatment in the soil. Culture-dependent and -independent evaluations revealed that MEP-degrading Burkholderia bacteria are predominant in soils after MEP application, limited members of which might play a pivotal role in MEP-degradation in soils. Notably, deep sequencing also revealed that some methylotrophs dramatically increased after MEP application, strongly suggesting that these bacteria play a role in the consumption and removal of methanol, a harmful derivative from MEP-degradation, for better growth of MEP-degrading bacteria. This comprehensive study demonstrated the succession and adaptation processes of microbial communities under MEP application, which were critically affected by past experience of insecticide-spraying.
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DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.4
作者：
Itoh, Hideomi;Aita, Manabu;Kikuchi, Yoshitomo
通讯作者：
Kikuchi, Yoshitomo
宏基因组分析揭示了淡水和海洋沉积物中持久性农药的潜在生物降解途径
影响因子：
9.8
作者：
Fang, Hua;Cai, Lin;Zhang, Tong
通讯作者：
Zhang, Tong
影响因子：
4.9
作者：
Kuklinsky-Sobral, HL;Araújo, WL;Azevedo, JL
通讯作者：
Azevedo, JL
影响因子：
3
作者：
Meyer, F.;Paarmann, D.;Edwards, R. A.
通讯作者：
Edwards, R. A.