Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa.
Genotyping-by-Sequencing-Based Genetic Analysis of African Rice Cultivars and Association Mapping of Blast Resistance Genes Against Magnaporthe oryzae Populations in Africa.
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非洲水稻品种基于测序的基因分型遗传分析和非洲稻瘟病群体稻瘟病抗性基因的关联作图。
DOI：
10.1094/phyto-12-16-0421-r
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发表时间：
2017
期刊：
影响因子：
3.2
通讯作者：
Mgonja EM
中科院分区：
文献类型：
--
作者：
Mgonja EM
了解水稻种质资源的遗传多样性对遗传物质在水稻育种和生产中的可持续利用具有重要意义。非洲拥有丰富的水稻遗传资源，可用于提高非洲大陆的水稻生产力。稻瘟病是制约非洲水稻生产的一个主要因素，稻瘟病是由半生物营养真菌病原稻瘟病菌引起的。在这篇报告中，我们介绍了基于基因分型测序（GBS）的190个非洲水稻品种的多样性分析结果，以及稻瘟病抗性(R)基因与数量性状位点（qtl）的关联图谱。根据GBS产生的184K单核苷酸多态性，将190个非洲品种聚为3组。我们给这些水稻品种接种了6种非洲菌。oryzaeisolates。关联图谱鉴定了水稻基因组中与稻瘟病抗性（RABRs）相关的25个基因组区域。此外，PCR分析表明rabr_23与12号染色体上的pi -基因相关。我们的研究表明，基于gbs的遗传多样性群体分析和关联定位相结合，可以有效地鉴定水稻稻瘟病R基因/ qtl，这些基因/ qtl有助于抵抗非洲稻瘟菌群体。oryzae。本研究鉴定的与rabr和14个高抗性品种相关的标记将为非洲水稻育种提供参考。
Understanding the genetic diversity of rice germplasm is important for the sustainable use of genetic materials in rice breeding and production. Africa is rich in rice genetic resources that can be utilized to boost rice productivity on the continent. A major constraint to rice production in Africa is rice blast, caused by the hemibiotrophic fungal pathogenMagnaporthe oryzae. In this report, we present the results of a genotyping-by-sequencing (GBS)-based diversity analysis of 190 African rice cultivars and an association mapping of blast resistance (R) genes and quantitative trait loci (QTLs). The 190 African cultivars were clustered into three groups based on the 184K single nucleotide polymorphisms generated by GBS. We inoculated the rice cultivars with six AfricanM. oryzaeisolates. Association mapping identified 25 genomic regions associated with blast resistance (RABRs) in the rice genome. Moreover, PCR analysis indicated thatRABR_23is associated with thePi-tagene on chromosome 12. Our study demonstrates that the combination of GBS-based genetic diversity population analysis and association mapping is effective in identifying rice blast R genes/QTLs that contribute to resistance against African populations ofM. oryzae. The identified markers linked to the RABRs and 14 highly resistant cultivars in this study will be useful for rice breeding in Africa.
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植物生物技术的未来
影响因子：
4.4
作者：
C. Stewart;D. Ow
通讯作者：
D. Ow
非洲水稻和相关野生物种的多样性。
影响因子：
5.4
作者：
K. Sanni;D. Tia;D. K. Ojo;A. Ogunbayo;M. Sikirou;N. S. Hamilton;M. Wopereis;D. Johnson;N. Ahmadi;E. Tollens;A. Jalloh
通讯作者：
A. Jalloh
影响因子：
3.2
作者：
Mundt, Christopher C.
通讯作者：
Mundt, Christopher C.
影响因子：
3.9
作者：
HIRANO, HY;MOCHIZUKI, K;SANO, Y
通讯作者：
SANO, Y