Mouse 230-kDa bullous pemphigoid antigen gene: structural and functional characterization of the 5'-flanking region and interspecies conservation of the deduced amino-terminal peptide sequence of the protein.
Mouse 230-kDa bullous pemphigoid antigen gene: structural and functional characterization of the 5'-flanking region and interspecies conservation of the deduced amino-terminal peptide sequence of the protein.
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小鼠 230-kDa 大疱性类天疱疮抗原基因：5 侧翼区域的结构和功能表征以及推导的蛋白质氨基末端肽序列的种间保守性。
DOI：
10.1111/1523-1747.ep12398405
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Uitto,J
中科院分区：
文献类型：
--
作者：
Sawamura,D;Sato,T;Kon,A;Harada,K;Nomura,K;Hashimoto,I;Tamai,K;Uitto,J
230 kDa大疱性类天疱疮抗原是皮肤基底膜区的半桥粒蛋白。从全长人cDNA推导的一级序列预测该分子由中心杆区域和侧翼球状结构域组成。为了深入了解230 kDa大疱性类天疱疮抗原基因（BPAG 1）的调控，并评估该蛋白氨基端的进化保守性，我们筛选了一个小鼠基因组DNA文库，该文库含有与人230 kDa大疱性类天疱疮抗原cDNA 5'端对应的0.3 kb cDNA。分离出阳性克隆，用0.3-kb cDNA对该克隆进行Southern分析，分离出含有编码序列5 ′端的3.0-kb Hind Ⅲ片段。该亚克隆的序列与人BPAG 1序列的比对显示，该片段含有ATG翻译起始位点上游的2466 bp的5 ′-侧翼DNA和258 bp的可翻译序列，该序列编码蛋白质氨基末端的86个氨基酸的推定多肽。该多肽序列与人源序列同源性为91%。TATAAA和CCAAT共有序列，以及几个假定的调控元件，在小鼠DNA的5 '侧翼区被鉴定。为了测试5 ′-侧翼DNA的功能性启动子活性，开发了三种小鼠BPAG 1启动子/CAT报告基因构建体，其启动子片段跨越-1133、-525和-213至-1。用这些构建体瞬时转染小鼠转化的角质形成细胞（Pam 212细胞）显示出可清楚检测的CAT活性，表明5 '侧翼区含有功能性启动子。此外，这些实验表明，上游序列含有上调元件，以及元件，赋予，至少部分，组织特异性的小鼠230-kDa BPA基因的表达。
The 230-kDa bullous pemphigoid antigen is a hemidesmosomal protein of the cutaneous basement membrane zone. The primary sequences deduced from full-length human cDNAs predict that this molecule consists of a central rod region and flanking globular domains. To get insight into regulation of the 230-kDa bullous pemphigoid antigen gene (BPAG1), and to evaluate evolutionary conservation of the amino-terminus of the protein, we screened a mouse genomic DNA library with a 0.3-kb cDNA corresponding to the 5' end of the human 230-kDa bullous pemphigoid antigen cDNA. A positive clone was isolated, and Southern analysis of the clone with the 0.3-kb cDNA allowed isolation of a 3.0-kb Hind III fragment containing the 5' end of the coding sequence. Alignment of the sequences of this subclone and human BPAG1 sequences revealed that this fragment contained 2466 bp of 5'- flanking DNA, upstream from the ATG translation initiation site, and 258 bp of translatable sequences that encode a putative polypeptide of 86 amino acids at the amino-terminus of the protein. This deduced polypeptide showed 91% homology with the corresponding human sequence. The TATAAA and CCAAT consensus sequences, as well as several putativecis-regulatory elements, were identified in the 5'-flanking region of the mouse DNA. To test the functional promoter activity of the 5'-flanking DNA, three mouse BPAG1 promoter/CAT reporter gene constructs, with the promoter segments spanning from -1133, -525, and -213 to -1, were developed. Transient transfections of mouse transformed keratinocytes (Pam 212 cells) with these constructs revealed clearly detectable CAT activities, indicating that the 5'-flanking region contains a functional promoter. Furthermore, these experiments suggested that the upstream sequences contain upregulatory elements, as well as elements that confer, at least in part, tissue specificity to the expression of the mouse 230-kDa BPA gene.
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人类 230-kD 大疱性类天疱疮抗原基因 (BPAG1)。
DOI：
10.1172/jci116655
发表时间：
1993
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Tamai,K;Sawamura,D;Do,HC;Tamai,Y;Li,K;Uitto,J
通讯作者：
Uitto,J
大疱性类天疱疮抗原 (BPAG1)：该基因的 cDNA 克隆和定位到人类 6 号染色体的短臂。
影响因子：
4.4
作者：
Sawamura,D;Nomura,K;Sugita,Y;Mattei,MG;Chu,ML;Knowlton,R;Uitto,J
通讯作者：
Uitto,J
体外控制小鼠黑色素瘤细胞色素的产生
影响因子：
7.8
作者：
S. Silagi
通讯作者：
S. Silagi
影响因子：
2.9
作者：
OWARIBE, K;KARTENBECK, J;FRANKE, WW
通讯作者：
FRANKE, WW
影响因子：
10.5
作者：
LEASK, A;ROSENBERG, M;FUCHS, E
通讯作者：
FUCHS, E