Structure-inherent targeting of near-infrared fluorophores for parathyroid and thyroid gland imaging.
Structure-inherent targeting of near-infrared fluorophores for parathyroid and thyroid gland imaging.
复制标题
用于甲状旁腺和甲状腺成像的近红外荧光团的结构固有靶向。
作者：
创建靶向造影剂的典型方法需要分离的靶向和荧光团结构域的共价结合。在这项研究中，我们证明了使用固有的化学结构来创建组织特异性近红外荧光团是可能的。因此，单个致密的分子既可以进行靶向，也可以进行成像。我们使用这一策略来解决头颈手术中的一个主要问题，即甲状旁腺和甲状腺的识别和保存。我们合成了7 0 0 nm和80 0 nm的卤代荧光团，它们在猪的单次静脉注射仅0.06 mg kg−1后，在特定的腺体中显示出高摄取。使用双通道近红外成像系统，我们展示了在血液和周围软组织的背景下，甲状旁腺和甲状腺的实时、高灵敏度、明确的识别。这项新技术为以更高的精度和效率进行头/颈手术奠定了基础，并潜在地降低了发病率，并为定向近红外荧光团的开发奠定了一般战略。
The typical method for creating targeted contrast agents requires covalent conjugation of separate targeting and fluorophore domains. In this study, we demonstrate that it is possible create tissue-specific near-infrared fluorophores using the inherent chemical structure. Thus, a single compact molecule performs both targeting and imaging. We use this strategy to solve a major problem in head/neck surgery, the identification and preservation of parathyroid and thyroid glands. We synthesized 700-nm and 800-nm halogenated fluorophores that show high uptake in the specific glands after a single intravenous injection of only 0.06 mg kg−1 in a pig. Using a dual-channel near-infrared imaging system, we demonstrate the real-time, high-sensitivity, unambiguous identification of parathyroid and thyroid glands simultaneously in the context of blood and surrounding soft tissue. This novel technology lays the foundation for head/neck surgery performed with increased precision and efficiency, and potentially lowers morbidity, and a general strategy for targeted near-infrared fluorophore development.
登录
查看更多内容
影响因子：
3.8
作者：
McWade, Melanie A.;Paras, Constantine;White, Lisa M.;Phay, John E.;Mahadevan-Jansen, Anita;Broome, James T.
通讯作者：
Broome, James T.
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru
影响因子：
2.6
作者：
Bliss, RD;Gauger, PG;Delbridge, LW
通讯作者：
Delbridge, LW
影响因子：
16.6
作者：
Achilefu, Samuel
通讯作者：
Achilefu, Samuel
影响因子：
2.8
作者：
Gibbs-Strauss, Summer L.;Nasr, Khaled A.;Frangioni, John V.
通讯作者：
Frangioni, John V.