Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.
Kindlin-2 expression in arsenite- and cadmium-transformed bladder cancer cell lines and in archival specimens of human bladder cancer.
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DOI：
10.1016/j.urology.2011.02.040
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发表时间：
2011-06
期刊：
影响因子：
2.1
通讯作者：
Sens DA
中科院分区：
文献类型：
--
作者：
Talaat S;Somji S;Toni C;Garrett SH;Zhou XD;Sens MA;Sens DA
这项研究的目的是证实一项微阵列研究，该研究表明Kindlin-2可能在膀胱癌的发展和进展中发挥作用。以前没有人膀胱癌中Kindlin-2表达的检查。采用真实的时间PCR、Western分析和免疫组织化学的组合来表征Kindlin-2在砷（As+3）和镉（Cd+2）转化的人细胞系、其在免疫受损小鼠中的肿瘤移植物以及人膀胱和膀胱癌的存档标本中的表达。结果表明，Kindlin-2在细胞系中的表达模式在肿瘤组织中不重复。然而，研究表明Kindlin-2在所有移植肿瘤和人膀胱癌存档标本的基质成分中表达。研究还表明，少数高级别浸润性尿路上皮癌在肿瘤细胞中具有Kindlin-2的灶性表达。Kindlin-2在大多数（如果不是全部）人膀胱癌的基质组分中表达。Kindlin-2在正常尿路上皮中不表达。Kindlin-2在一小部分高级别浸润性膀胱癌中表达，可能作为肿瘤进展的预后标志物。
The goal of this study was to confirm a microarray study that suggested that Kindlin-2 might play a role in the development and progression of bladder cancer. There has been no previous examination of Kindlin-2 expression in human bladder cancer. A combination of real time PCR, western analysis and immunohistochemistry was used to characterize Kindlin-2 expression in arsenite (As+3) and cadmium (Cd+2) transformed human cell lines, their tumor transplants in immune-compromised mice, and in archival specimens of human bladder and bladder cancer. The results show that the Kindlin-2 expression patterns in the cell lines were not duplicated in the tumor tissues. However, it was shown that Kindlin-2 was expressed in the stromal element of all the transplanted tumors and archival specimens of human bladder cancer. It was also shown that a small number of high grade invasive urothelial cancers have focal expression of Kindlin-2 in the tumor cells. Kindlin-2 is expressed in the stromal component of most, if not all, human bladder cancers. Kindlin-2 is not expressed in normal urothelium. Kindlin-2 is expressed in a small subset of high grade invasive bladder cancers and may have potential as a prognostic marker for tumor progression.
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影响因子：
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作者：
Kellen, Eliane;Zeegers, Maurice P.;Buntinx, Frank
通讯作者：
Buntinx, Frank
影响因子：
5
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影响因子：
2.4
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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