Sesamin protects SH-SY5Y cells against mechanical stretch injury and promoting cell survival.
Sesamin protects SH-SY5Y cells against mechanical stretch injury and promoting cell survival.
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芝麻素保护 SH-SY5Y 细胞免受机械拉伸损伤并促进细胞存活
DOI：
10.1186/s12868-017-0378-8
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发表时间：
2017-08-07
期刊：
影响因子：
2.4
通讯作者：
Tian H
中科院分区：
文献类型：
--
作者：
Xu Z;Liu Y;Yang D;Yuan F;Ding J;Chen H;Tian H
研究背景芝麻素是一种从芝麻中提取的抗氧化剂，具有多种疗效。本研究探讨了芝麻素是否会保护神经母细胞瘤SH-SY 5 Y细胞免受机械牵张损伤诱导的活性氧（ROS）和凋亡增加。此外，研究了这些活性物质的潜在机制。在暴露于机械拉伸损伤后，将细胞孵育用于进一步研究。使用乳酸脱氢酶和Cell Counting Kit-8测定法评估细胞活力，并进行末端脱氧核苷酸转移酶dUTP缺口末端标记测定法和流式细胞术分析，以评价线粒体膜电位（Δ Km）的变化。此外，通过20，70-二氯荧光素二乙酸盐染色测量细胞内ROS产生水平，使用实时聚合酶链反应分析评估基质金属肽酶9（MMP-9）的mRNA水平，结果机械牵张损伤可显著降低细胞活力，但预处理可减轻这种影响芝麻素（50 μM）。芝麻素还显著抑制机械牵张损伤诱导的细胞内ROS产生的增加，减弱Δ λ m的下降，减少促凋亡蛋白的表达，并减少细胞凋亡。牵张损伤增加Bax和切割的caspase 3水平，增强MMP-9的基因表达，增加Akt、p38和JNK的磷酸化水平，并降低细胞中Bcl-2水平。芝麻素预处理可降低机械牵张损伤诱导的MMP-9的表达。结论芝麻素通过抑制细胞内ROS水平的升高和抑制细胞凋亡，保护SH-SY 5 Y细胞免受牵张损伤。因此，芝麻素似乎是治疗创伤性脑损伤的潜在治疗剂。
BackgroundSesamin is a well-known antioxidant extracted from sesame seeds that exhibits various curative effects. The present study investigated whether sesamin would protect neuroblastoma SH-SY5Y cells against mechanical stretch injury-induced increases in reactive oxygen species (ROS) and apoptosis. Additionally, the mechanisms underlying these actives were investigated. Following exposure to mechanical stretch injury, cells were incubated for further investigations. Lactate dehydrogenase and Cell Counting Kit-8 assays were used to assess cell viability, and a terminal deoxynucleotidyl transferase dUTP nick end labeling assay and flow cytometric analysis were performed to evaluate changes in mitochondrial membrane potential (ΔΨm). Furthermore, intracellular levels of ROS production were measured by 20, 70-dichlorofluorescein diacetate staining, the mRNA levels of matrix metallopeptidase 9 (MMP-9) were evaluated using real-time polymerase chain reaction analysis, and the determinations had also been made on related proteins by Western blot analysis.ResultsExposure to mechanical stretch injury significantly decreased cell viability but this decrease was attenuated by pretreatment with sesamin (50 μM). Sesamin also significantly inhibited mechanical stretch injury-induced increases in intracellular ROS production, attenuated declines in ΔΨm, diminished the expressions of pro-apoptotic proteins, and decreased cell apoptosis. Stretch injury increased Bax and cleaved caspase 3 levels, enhanced the gene expression of MMP-9, increased the phosphorylation levels of Akt, p38, and JNK and decreased Bcl-2 levels in the cells. However, pretreatment with sesamin reduced the mechanical stretch injury-induced overexpression of MMP-9.ConclusionsSesamin protected SH-SY5Y cells against stretch injury by attenuating increases in ROS levels and suppressing apoptosis. Accordingly, sesamin seems to be a potentially therapeutic agent in the treatment of traumatic brain injury.
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影响因子：
4.7
作者：
Ahmed, SM;Rzigalinski, BA;Ellis, EF
通讯作者：
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影响因子：
2.5
作者：
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通讯作者：
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影响因子：
4.5
作者：
Galati, G;Sabzevari, O;O'Brien, PJ
通讯作者：
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影响因子：
2.5
作者：
Song, Hai;Xu, Lincheng;Yu, Jianyun
通讯作者：
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影响因子：
4.2
作者：
DIETRICH, WD;ALONSO, O;HALLEY, M
通讯作者：
HALLEY, M