Maternal cold exposure induces distinct transcriptome changes in the placenta and fetal brown adipose tissue in mice.
Maternal cold exposure induces distinct transcriptome changes in the placenta and fetal brown adipose tissue in mice.
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DOI：
10.1186/s12864-021-07825-6
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发表时间：
2021-07-03
期刊：
影响因子：
4.4
通讯作者：
Chang JS
中科院分区：
文献类型：
--
作者：
Ghosh S;Park CH;Lee J;Lee N;Zhang R;Huesing C;Reijnders D;Sones J;Münzberg H;Redman L;Chang JS
棕色脂肪组织（BAT）专门以热量的形式耗散能量。BAT介导的啮齿动物和人类产热对于新生儿出生后立即有效适应子宫外环境的温度至关重要。然而，很少有人知道胎儿BAT的发展是否以及如何在子宫内调制响应于怀孕期间母体热环境的变化。使用BL 6小鼠，我们评估了不同母体环境温度（28 °C和18 °C）对胎盘和胎儿BAT的转录组的影响，以测试母体冷暴露是否通过胎盘重塑影响胎儿BAT发育。在28 °C和18 °C下，妊娠期间母体体重增加、每次妊娠的平均胎儿数量和胎盘重量在两组之间没有差异。然而，与28 ° C组相比，18 ° C组在E18.5时的平均胎仔体重低6%。在胎儿BAT中，妊娠期间的冷暴露诱导参与从头脂肪生成和脂质代谢的基因的表达增加，同时降低与肌细胞分化相关的基因的表达，从而表明母体冷暴露可能通过抑制双向祖细胞中的肌源性谱系来促进胎儿棕色脂肪生成。在胎盘组织中，母体冷暴露与参与补体激活的基因上调和与肌肉收缩和肌动蛋白-肌球蛋白丝滑动相关的基因下调有关。这些变化可能通过保护免受冷应激诱导的炎症损伤和调节胎盘中的血管和血管外收缩系统来协调胎盘对母体冷暴露的适应。这些发现提供了证据表明，母亲感受到的环境寒冷温度可以调节胎盘和胎儿BAT组织的转录组。观察到的基因表达变化的后果值得进一步研究。在线版本包含补充材料，可通过10.1186/s12864-021-07825-6获得。
Brown adipose tissue (BAT) is specialized to dissipate energy in the form of heat. BAT-mediated heat production in rodents and humans is critical for effective temperature adaptation of newborns to the extrauterine environment immediately after birth. However, very little is known about whether and how fetal BAT development is modulated in-utero in response to changes in maternal thermal environment during pregnancy. Using BL6 mice, we evaluated the impact of different maternal environmental temperatures (28 °C and 18 °C) on the transcriptome of the placenta and fetal BAT to test if maternal cold exposure influences fetal BAT development via placental remodeling. Maternal weight gain during pregnancy, the average number of fetuses per pregnancy, and placental weight did not differ between the groups at 28 °C and 18 °C. However, the average fetal weight at E18.5 was 6% lower in the 18 °C-group compared to the 28 °C-group. In fetal BATs, cold exposure during pregnancy induced increased expression of genes involved in de novo lipogenesis and lipid metabolism while decreasing the expression of genes associated with muscle cell differentiation, thus suggesting that maternal cold exposure may promote fetal brown adipogenesis by suppressing the myogenic lineage in bidirectional progenitors. In placental tissues, maternal cold exposure was associated with upregulation of genes involved in complement activation and downregulation of genes related to muscle contraction and actin-myosin filament sliding. These changes may coordinate placental adaptation to maternal cold exposure, potentially by protecting against cold stress-induced inflammatory damage and modulating the vascular and extravascular contractile system in the placenta. These findings provide evidence that environmental cold temperature sensed by the mother can modulate the transcriptome of placental and fetal BAT tissues. The ramifications of the observed gene expression changes warrant future investigation. The online version contains supplementary material available at 10.1186/s12864-021-07825-6.
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影响因子：
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