Developmentally Regulated Splicing of the Third Intron of P Element in Somatic Tissues in Drosophila Embryos
Developmentally Regulated Splicing of the Third Intron of P Element in Somatic Tissues in Drosophila Embryos
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果蝇胚胎体组织中 P 元件第三内含子的发育调控剪接
DOI：
10.1111/j.1440-169x.1993.00067.x
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发表时间：
1993
期刊：
影响因子：
4.6
通讯作者：
M. Okada
中科院分区：
文献类型：
--
作者：
T. Kitamura;Satoru Kobayashi;M. Okada
在果蝇中，有人认为P元件的第三个内含子仅在生殖细胞中被剪接掉。然而，我们发现剪接发生在胚胎发生过程中的一些体细胞组织，以及极细胞，生殖系的祖细胞。为了检测体内剪接活性，我们设计了一种测定系统，通过β-半乳糖苷酶活性的组织化学染色来可视化第三内含子被剪接掉的细胞。在本文中，我们描述了体细胞组织中的第三个内含子剪接在胚胎发生过程中，参考剪接发生的阶段。基于这些发现，我们提出了对先前文献的修改，即来自P元件的转录本的完整加工仅发生在生殖系细胞中。
In Drosophila, it has been suggested that the third intron of the P element is spliced out only in germ‐line cells. However, we found that the splicing took place in some somatic tissues during embryogenesis, as well as in pole cells, progenitor cells of the germ line. To detect the splicing activity in vivo, we designed an assay system to visualize the cells where the third intron was spliced out by the histochemical staining for β‐galactosidase activity. In this paper, we describe the somatic tissues where the third intron is spliced out during embryogenesis, with reference to the stages when the splicing occurs. Based on these findings we propose a modification of the previous documentation that the complete processing of transcripts from P element takes place only in germ‐line cells.
影响因子：
11.1
作者：
D. Rio
通讯作者：
D. Rio
鉴定和纯化与 P 转座元件末端 31 个碱基对反向重复序列结合的果蝇蛋白。
DOI：
10.1073/pnas.85.23.8929
发表时间：
1988
影响因子：
11.1
作者：
Rio,DC;Rubin,GM
通讯作者：
Rubin,GM
影响因子：
11.4
作者：
ADAMI, G;BABISS, LE
通讯作者：
BABISS, LE