Hemocyanin genes as indicators of habitat shifts in Panpulmonata?

Hemocyanin genes as indicators of habitat shifts in Panpulmonata?
复制标题

血蓝蛋白基因作为 Panpulmonata 栖息地变化的指标?

DOI:
10.1016/j.ympev.2018.10.014
复制
发表时间:
2019
影响因子:
4.1
通讯作者:
Lieb B
Lieb B
中科院分区:
生物学1区
文献类型:
--
作者:
Schäfer GG;Pedrini-Martha V;Schnegg R;Dallinger R;Jackson DJ;Lieb B

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血蓝蛋白是大多数软体动物的主要呼吸蛋白,因此直接与每个物种的生理需求及其适应的环境相互作用。因此,血蓝蛋白很可能是由显著的栖息地变化而在进化上留下的印记。在腹足类分支Panpulmonata(> 30,000种)中,主要的领域转换已经独立地发生了多次,并且可能有助于这个群体的多样化。然而,人们对与这些栖息地变化有关的适应性变化知之甚少。为了更深入地了解全肺血蓝蛋白的进化,并推断与这些情况相关的可能影响,我们已经组装和分析了血蓝蛋白亚型从4个全肺物种:(i)Hellopomatia,(ii)Cantareus aspersus(两个螺旋科,Stylommatophora),(iii)Arion vulgaris(Arionidae,Stylommatophora)和(iv)Stagnaea stagnalis(Ammaeidae,Hygrophila)。此外,我们描述了一个新的血蓝蛋白亚型的基因组内的euopisthobranchAussia californica.Using这些新获得的血蓝蛋白的数据,我们进行了系统发育分析,揭示了独立的复制事件的血蓝蛋白的谱系内,与显着的栖息地的变化。
Hemocyanin is the primary respiratory protein for the majority of the Mollusca and therefore directly interfaces with the physiological requirements of each species and the environments to which they are adapted. Hemocyanin is therefore likely to have been evolutionarily imprinted by significant habitat shifts. In the gastropod clade Panpulmonata (>30,000 species) major realm transitions have occurred multiple times independently and may have contributed to the diversification of this group. Yet, little is known about the adaptive changes linked to these habitat shifts. In order to gain deeper insight into the evolution of panpulmonate hemocyanins and to infer possible impacts associated with those scenarios, we have assembled and analysed hemocyanin isoforms from 4 panpulmonate species: (i)Helix pomatia, (ii) Cantareus aspersus(both Helicidae, Stylommatophora), (iii)Arion vulgaris(Arionidae, Stylommatophora) and (iv)Lymnaea stagnalis(Lymnaeidae, Hygrophila). Additionally, we describe a new hemocyanin isoform within the genome of the euopisthobranchAplysia californica.Using these newly acquired hemocyanin data, we performed a phylogenetic analysis that reveals independent duplication events of hemocyanin within lineages that correlate with significant habitat shifts.
根据 DNA 测序推断软体动物血蓝蛋白的进化。
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