Cloning, characterization and expression of tyrosinase-like gene in amphioxus Branchiostoma japonicum.
Cloning, characterization and expression of tyrosinase-like gene in amphioxus Branchiostoma japonicum.
复制标题
文昌鱼酪氨酸酶样基因的克隆、鉴定及表达。
DOI：
10.1016/j.fsi.2012.11.013
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Bosheng Zhao
中科院分区：
文献类型：
--
作者：
Meng Zhang;Yun Bai;Lili Gao;Bosheng Zhao
原酚氧化酶（酪氨酸酶）广泛分布于无脊椎动物和脊椎动物体内，在先天免疫中起着至关重要的作用。本研究利用PCR技术从日本文昌鱼（amphioxus Branchiostoma japonicum）中克隆了一个酪氨酸酶样（命名为AmphiTYR）的全长cDNA。AmphiTYR全长2314 bp，其开放阅读框编码一个544个氨基酸的蛋白，预计分子量约为60.9 kDa，等电点为5.65。它在酪氨酸酶蛋白中有一个保守的假定的铜结合结构域，具有6个组氨酸。6个潜在的n链糖基化位点和14个保守的半胱氨酸残基也被预测存在于日本血吸虫酪氨酸酶中。同源性分析表明，AmphiTYR与脊椎动物酪氨酸酶（32.5 ~ 40.5%）的相似性高于与无脊椎动物酚氧化酶（6.4 ~ 25.4%）的相似性。在成虫的肌肉、表皮、脊索、卵巢、肝盲肠、咽和鳃中表达AmphiTYR mRNA，但在神经管、肠和睾丸中不表达。在文昌鱼从未受精卵到幼虫的不同发育阶段，AmphiTYR在文昌鱼的整个发育过程中都有表达。这些结果表明，AmphiTYR基因不仅在先天免疫中起着关键作用，而且在头脊索文昌鱼的胚胎发育过程中也起着重要作用。
Prophenoloxidase (tyrosinase) widely distributed in invertebrates and vertebrates, and plays a crucial role in the innate immune. In the present study, the full-length cDNA of a tyrosinase-like (designated AmphiTYR) was cloned from amphioxus Branchiostoma japonicum by PCR techniques. The full-length cDNA of AmphiTYR is 2314 bp, and its predicted open-reading frame codes for a protein of 544 amino acids with a predicted molecular mass of approximately 60.9 kDa and an isoelectric point of 5.65. It has a conserved putative copper-binding domain with six histidines in tyrosinase proteins. Six potential N-linked glycosylation sites and 14 conserved cysteine residues were also predicted to be present in B. japonicum tyrosinase. Homology analysis revealed that AmphiTYR was higher similar to vertebrates tyrosinases (32.5–40.5%) than to invertebrates phenoloxidase (6.4–25.4%). In the adult, AmphiTYR mRNA was expressed in the muscle, epidermis, notochord, ovary, hepatic caecum, pharynx and gill, but not in the neural tube, intestines and testis. During the different development stages from unfertilized egg to larvae of amphioxus, AmphiTYR expressed during all the amphioxus development. These results indicated that AmphiTYR gene not only play a pivotal role in innate immune but also play an important role during embryonic development of cephalochordate amphioxus.
登录
查看更多内容
DOI：
10.1111/j.1600-0749.1998.tb00737.x
发表时间：
1998-10
期刊：
Pigment cell research
影响因子：
--
作者：
H. Inagaki;A. Koga;Y. Bessho;H. Hori
通讯作者：
H. Inagaki;A. Koga;Y. Bessho;H. Hori
影响因子：
3.4
作者：
Giménez, E;Lavado, A;Montoliu, L
通讯作者：
Montoliu, L
影响因子：
2.9
作者：
C. Ko;Tzu-Ting Chiou;B. Vaseeharan;Jenn-Kan Lu;Jiann-Chu Chen
通讯作者：
C. Ko;Tzu-Ting Chiou;B. Vaseeharan;Jenn-Kan Lu;Jiann-Chu Chen
影响因子：
2.5
作者：
Schmutz, SM;Berryere, TG;Fredholm, M
通讯作者：
Fredholm, M
DOI：
10.1111/j.1600-0749.1993.tb00622.x
发表时间：
1993-12
期刊：
Pigment cell research
影响因子：
--
作者：
Zit Abattyani;Luc Xerri;Jacques Hassoun;Bonérandi Jj;Jean-Jacques Grob
通讯作者：
Zit Abattyani;Luc Xerri;Jacques Hassoun;Bonérandi Jj;Jean-Jacques Grob