A Model of Tracer Transport in Airway Surface Liquid
A Model of Tracer Transport in Airway Surface Liquid
复制标题
气道表面液体示踪剂传输模型
DOI：
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发表时间：
2007
影响因子：
3.5
通讯作者：
John Blake
中科院分区：
文献类型：
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作者：
David J. Smith;E. Gaffney;John Blake
本研究关注的是调和理论建模的流体流动在气道表面液体与实验可视化示踪剂运输在人类气道上皮细胞培养。气道被一层浓密的纤毛覆盖，纤毛长度约为1.6 μm，在含水的纤毛周液（PCL）中跳动。在此之上有一层粘弹性粘液，它捕获吸入的病原体。纤毛推动粘液沿着气道流向气管和口腔。节拍周期的理论分析smithd，fulb预测PCL的运输量与粘液相比较小，这是基于上皮不渗透液体的假设。然而，一项实验研究表明PCL和粘液的转运几乎相等。基于对ASL中稳定对流-扩散的现有理解（Blake和Gaffney，2001年; Mitran，2004年），使用示踪剂输送的对流-扩散模型的数值模拟来测试几种拟议的流动剖面，并测试由跳动纤毛引起的振荡剪切的重要性。机械衍生的振荡流与PCL的非常低的平均运输结果在相对较小的示踪剂脉冲的“涂抹”。其他的效果，如PCL和粘液之间的混合，以及在纤毛尖端上方的PCL的上部中的显著运输进行了测试，并导致更紧密的运输，两层中的示踪剂脉冲之间的分离小于9%。此外，如果PCL的平均转运仅为粘液转运的50%，显著小于基于实验结果首先推断的平均PCL转运，则实验结果可以被复制到非常高的准确度。
This study is concerned with reconciling theoretical modelling of the fluid flow in the airway surface liquid with experimental visualisation of tracer transport in human airway epithelial cultures. The airways are covered by a dense mat of cilia of length ∼ 6 μm beating in a watery periciliary liquid (PCL). Above this there is a layer of viscoelastic mucus which traps inhaled pathogens. Cilia propel mucus along the airway towards the trachea and mouth. Theoretical analyses of the beat cycle smithd, fulb predict small transport of PCL compared with mucus, based on the assumption that the epithelium is impermeable to fluid. However, an experimental study coord indicates nearly equal transport of PCL and mucus. Building on existing understanding of steady advection-diffusion in the ASL (Blake and Gaffney, 2001; Mitran,2004) numerical simulation of an advection-diffusion model of tracer transport is used to test several proposed flow profiles and to test the importance of oscillatory shearing caused by the beating cilia. A mechanically derived oscillatory flow with very low mean transport of PCL results in relatively little ‘smearing’ of the tracer pulses. Other effects such as mixing between the PCL and mucus, and significant transport in the upper part of the PCL above the cilia tips are tested and result in still closer transport, with separation between the tracer pulses in the two layers being less than 9%. Furthermore, experimental results may be replicated to a very high degree of accuracy if mean transport of PCL is only 50% of mucus transport, significantly less than the mean PCL transport first inferred on the basis of experimental results.
影响因子：
15.9
作者：
Matsui, H;Randell, SH;Boucher, RC
通讯作者：
Boucher, RC