Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins.
Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins.
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目前的趋势和挑战，快速智能诊断点的网站测试海洋毒素。
DOI：
10.3390/s21072499
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发表时间：
2021-04-03
期刊：
影响因子：
--
通讯作者：
Campbell K
中科院分区：
文献类型：
--
作者：
Dillon M;Zaczek-Moczydlowska MA;Edwards C;Turner AD;Miller PI;Moore H;McKinney A;Lawton L;Campbell K
在过去的二十年中，在欧洲（EU）和其他地区，常规监管监测中的海洋生物毒素分析已经从使用小鼠生物测定法（MBA）向高端分析技术（如高效液相色谱法（HPLC）和串联质谱法（MS））发展。以前，接受这些先进的方法，在进步远离MBA，是阻碍了缺乏商业认证的分析标准的方法开发和验证。这一问题现在已经得到解决，欧盟、北美和亚洲几家公司提供的各种分析标准品的可用性加强了方法的开发和验证，使其符合所需的监管标准。然而，高端分析设备的成本、冗长的程序以及对合格人员进行分析的需要，仍然是常规监测实验室面临的挑战。在发展中地区，水产养殖产量正在增加，替代性廉价的灵敏、可测量、准确和实时（SMART）快速现场测试（POST）方法适合于新手最终用户，可以验证并得到国际认可，仍然是监管机构和行业的目标。市场上用于海洋毒素分析的商业检测试剂盒的范围仍然有限，满足监管要求的试剂盒更是如此。单个检测包括酶联免疫吸附测定（ELISA）和基于侧流膜的免疫测定（LFIA），用于欧盟监管的毒素，如冈田酸（OA）和甲藻毒素（DTX）、石房蛤毒素（STX）及其类似物和软骨藻酸（DA），以三种独立的检测形式提供不同的成本和行业利益。从文献中可以观察到，不仅这些测定法正在进行开发和改进，而且还使用即将到来的最先进的生物传感器技术开发了新的测定法。本综述着重于海洋生物毒素测试的现有方法和创新方法的最新进展，以及需要观察的最终用户实用性。此外，它还强调了影响检测发展的趋势，如多路复用能力和快速POST，表明了将塑造未来市场的潜在检测方法。
In the past twenty years marine biotoxin analysis in routine regulatory monitoring has advanced significantly in Europe (EU) and other regions from the use of the mouse bioassay (MBA) towards the high-end analytical techniques such as high-performance liquid chromatography (HPLC) with tandem mass spectrometry (MS). Previously, acceptance of these advanced methods, in progressing away from the MBA, was hindered by a lack of commercial certified analytical standards for method development and validation. This has now been addressed whereby the availability of a wide range of analytical standards from several companies in the EU, North America and Asia has enhanced the development and validation of methods to the required regulatory standards. However, the cost of the high-end analytical equipment, lengthy procedures and the need for qualified personnel to perform analysis can still be a challenge for routine monitoring laboratories. In developing regions, aquaculture production is increasing and alternative inexpensive Sensitive, Measurable, Accurate and Real-Time (SMART) rapid point-of-site testing (POST) methods suitable for novice end users that can be validated and internationally accepted remain an objective for both regulators and the industry. The range of commercial testing kits on the market for marine toxin analysis remains limited and even more so those meeting the requirements for use in regulatory control. Individual assays include enzyme-linked immunosorbent assays (ELISA) and lateral flow membrane-based immunoassays (LFIA) for EU-regulated toxins, such as okadaic acid (OA) and dinophysistoxins (DTXs), saxitoxin (STX) and its analogues and domoic acid (DA) in the form of three separate tests offering varying costs and benefits for the industry. It can be observed from the literature that not only are developments and improvements ongoing for these assays, but there are also novel assays being developed using upcoming state-of-the-art biosensor technology. This review focuses on both currently available methods and recent advances in innovative methods for marine biotoxin testing and the end-user practicalities that need to be observed. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid POST, indicating potential detection methods that will shape the future market.
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DOI：
10.1080/19440049.2010.531198
发表时间：
2011-06
期刊：
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment
影响因子：
--
作者：
Campbell K;Rawn DF;Niedzwiadek B;Elliott CT
通讯作者：
Elliott CT
影响因子：
6.6
作者：
Anderson DM;Alpermann TJ;Cembella AD;Collos Y;Masseret E;Montresor M
通讯作者：
Montresor M
影响因子：
6.1
作者：
Alonso, Eva;Alfonso, Amparo;Botana, Luis M.
通讯作者：
Botana, Luis M.
影响因子：
11.4
作者：
Boscolo, S.;Pelin, M.;Tubaro, A.
通讯作者：
Tubaro, A.
影响因子：
6.2
作者：
Campas, Monica;Marty, Jean-Louis
通讯作者：
Marty, Jean-Louis