Paralytic shellfish poisoning (PSP) toxin binders for optical biosensor technology: problems and possibilities for the future: a review.
Paralytic shellfish poisoning (PSP) toxin binders for optical biosensor technology: problems and possibilities for the future: a review.
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DOI：
10.1080/19440049.2010.531198
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发表时间：
2011-06
期刊：
影响因子：
--
通讯作者：
Elliott CT
中科院分区：
文献类型：
--
作者：
Campbell K;Rawn DF;Niedzwiadek B;Elliott CT
本文综述了利用表面等离子体共振现象检测麻风性贝毒(PSP)毒素的光学生物传感器技术的发展。光学生物传感器技术测量特定生物识别元件或粘合剂与固定在传感器芯片表面的目标毒素对样品中毒素的竞争生物分子相互作用。不同的结合剂，如以前在功能和免疫分析中使用的受体和抗体，已经进行了评估。重点强调了使用单一粘合剂检测这一系列低分子毒素的困难，因为类似物在四个化学取代部位不同。对于相对于小鼠生物测定的总毒性的测量，还考虑了每种毒素相对于母体化合物的毒性因素所引起的并发症。对于抗体，交叉反应曲线并不总是与毒力相关，而是与它所产生的毒素结构有关。毒素的限制和可获得性使得合成用于抗体生产的蛋白质结合衍生物的替代化学策略成为一项困难的任务。然而，当使用两种具有不同交叉反应曲线的抗体时，两种抗体的毒素芯片表面都是通用的，这表明每种抗体的交叉反应曲线都可以组合成一种单一的检测形式。讨论了用于光学生物传感器分析低分子化合物的受体的困难，以及替代非抗体为基础的结合剂在这一领域未来分析发展的潜力。
This review examines the developments in optical biosensor technology, which uses the phenomenon of surface plasmon resonance, for the detection of paralytic shellfish poisoning (PSP) toxins. Optical biosensor technology measures the competitive biomolecular interaction of a specific biological recognition element or binder with a target toxin immobilised onto a sensor chip surface against toxin in a sample. Different binders such as receptors and antibodies previously employed in functional and immunological assays have been assessed. Highlighted are the difficulties in detecting this range of low molecular weight toxins, with analogues differing at four chemical substitution sites, using a single binder. The complications that arise with the toxicity factors of each toxin relative to the parent compound, saxitoxin, for the measurement of total toxicity relative to the mouse bioassay are also considered. For antibodies, the cross-reactivity profile does not always correlate to toxic potency, but rather to the toxin structure to which it was produced. Restrictions and availability of the toxins makes alternative chemical strategies for the synthesis of protein conjugate derivatives for antibody production a difficult task. However, when two antibodies with different cross-reactivity profiles are employed, with a toxin chip surface generic to both antibodies, it was demonstrated that the cross-reactivity profile of each could be combined into a single-assay format. Difficulties with receptors for optical biosensor analysis of low molecular weight compounds are discussed, as are the potential of alternative non-antibody-based binders for future assay development in this area.
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影响因子：
5.1
作者：
Dell'Aversano, Carmela;Walter, John A.;Quilliam, Michael A.
通讯作者：
Quilliam, Michael A.
影响因子：
6.1
作者：
Chu, FS;Hsu, KH;Allison, C
通讯作者：
Allison, C
DOI：
10.1016/j.jchromb.2009.10.023
发表时间：
2009-12-15
影响因子：
3
作者：
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通讯作者：
Elliott, Christopher T.
影响因子：
3.1
作者：
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通讯作者：
Luckas, Bernd
影响因子：
7.4
作者：
Campbell, Katrina;Haughey, Simon A.;Elliott, Christopher T.
通讯作者：
Elliott, Christopher T.