Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity
Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity
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细菌磁性颗粒-聚乙烯亚胺载体将靶基因高效低毒地递送至多种细胞类型
DOI：
10.1007/s00253-020-10729-2
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发表时间：
2020-06
影响因子：
5
通讯作者：
Fang Meiying
中科院分区：
文献类型：
--
作者：
Yang Wanjie;Tang Qiguo;Bai Ying;Wang Kejun;Dong Xinxing;Li Ying;Fang Meiying
摘要细菌磁性颗粒（BMPs）是一种生物合成的磁性纳米材料，具有良好的吸附和生物膜封闭性能。在这项研究中，我们证明骨形态发生蛋白增强了聚乙烯亚胺（PEI）将靶DNA输送到难以转染的原代猪肝脏细胞中的能力，转染效率达到30%以上。与标准脂质体转染和多聚体转染相比，BMP-PEI基因载体对原代猪肝细胞和C2 C12小鼠成肌细胞的转染效率显著提高。为了更好地理解使用BMP-PEI/DNA载体的磁转染机制，观察转染的Cos-7、HeLa和HEP-G2细胞的透射电子显微镜（TEM）图像。我们发现BMP-PEI/DNA复合物通过囊泡运输和内吞作用进入细胞质和细胞核。我们的研究为多功能BMP-PEI载体转染系统提供了支持，该系统可用于广泛的细胞类型，甚至在疾病治疗中达到特定的靶点。关键点·我们通过将BMP和PEI结合构建了BMP-PEI基因递送载体。·该载体显著提高了真核细胞系的转染效率。·并对该载体的转染机制进行了初步的探讨。
AbstractBacterial magnetic particles (BMPs) are biosynthesized magnetic nano-scale materials with excellent dispersibility and biomembrane enclosure properties. In this study, we demonstrate that BMPs augment the ability of polyethylenimine (PEI) to deliver target DNA into difficult-to-transfect primary porcine liver cells, with transfection efficiency reaching over 30%. Compared with standard lipofection and polyfection, BMP-PEI gene vectors significantly enhanced the transfection efficiencies for the primary porcine liver cells and C2C12 mouse myoblast cell lines. To better understand the mechanism of magnetofection using BMP-PEI/DNA vectors, transmission electron microscopy (TEM) images of transfected Cos-7, HeLa, and HEP-G2 cells were observed. We found that the BMP-PEI/DNA complexes were trafficked into the cytoplasm and nucleus by way of vesicular transport and endocytosis. Our study builds support for the versatile BMP-PEI vector transfection system, which might be exploited to transfect a wide range of cell types or even to reach specific targets in the treatment of disease.Key Points• We constructed a BMP-PEI gene delivery vector by combining BMPs and PEI.• The vector significantly enhanced transfection efficiencies in eukaryotic cell lines.• The transfection mechanism of this vector was explained in our study.
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影响因子：
3.7
作者：
Chen, M;von Mikecz, A
通讯作者：
von Mikecz, A
影响因子：
17.1
作者：
Kievit FM;Veiseh O;Fang C;Bhattarai N;Lee D;Ellenbogen RG;Zhang M
通讯作者：
Zhang M
影响因子：
3.2
作者：
BALKWILL, DL;MARATEA, D;BLAKEMORE, RP
通讯作者：
BLAKEMORE, RP
影响因子：
17.1
作者：
Cheng, Jinping;Fernando, K. A. Shiral;Cheng, Shuk Han
通讯作者：
Cheng, Shuk Han
DOI：
10.1017/9781139381222.009
发表时间：
2019-02
期刊：
Magnetic Nanoparticles in Biosensing and Medicine
影响因子：
--
作者：
D. Bazylinski;Denis Trubitsyn
通讯作者：
D. Bazylinski;Denis Trubitsyn