Nanotechnology-assisted adipose-derived stem cell (ADSC) therapy for erectile dysfunction of cavernous nerve injury: In vivo cell tracking, optimized injection dosage, and functional evaluation.
Nanotechnology-assisted adipose-derived stem cell (ADSC) therapy for erectile dysfunction of cavernous nerve injury: In vivo cell tracking, optimized injection dosage, and functional evaluation.
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纳米技术辅助脂肪干细胞（ADSC）治疗海绵神经损伤性勃起功能障碍：体内细胞追踪、优化注射剂量和功能评估
DOI：
10.4103/aja.aja_48_18
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发表时间：
2018-09
影响因子：
2.9
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Wu H;Tang WH;Zhao LM;Liu DF;Yang YZ;Zhang HT;Zhang Z;Hong K;Lin HC;Jiang H
干细胞疗法是治疗勃起功能障碍的一个潜在的有希望的选择；然而，它的致瘤性风险是一个临床障碍，风险与注射细胞的数量呈正相关。我们之前的研究表明，纳米技术通过吸引海绵体中的细胞改善了脂肪源性干细胞（ADSC）治疗海绵体神经损伤（CNI）的勃起功能障碍。这些结果提示了使用减少剂量的ADSCs进行海绵内注射的可能性。在这项探索性研究中，我们使用低剂量（2 × 105个细胞）的ADSCs进行海绵体内注射（ICI）和纳米技术方法。在第28天测量海绵内压和平均动脉压以评估勃起功能。低剂量ADSC治疗组表现出良好的治疗效果，纳米技术进一步改善了这些效果。在ICI细胞的体内成像显示，nanoshuttle结合的ADSCs （NanoADSCs）在第0、1和3天的荧光素信号比ADSCs强得多。免疫荧光和Western blot分析显示，与CNI组相比，ADSC组的平滑肌、内皮细胞和神经组织明显增加；在辅助纳米技术的帮助下，进一步改进。这些发现表明，纳米技术可以进一步提高小剂量ADSCs改善勃起功能的效果。大量的纳米adscs在体内海绵体中保留至少3天。勃起功能改善的机制可能与平滑肌、内皮细胞和神经组织的再生有关。
Stem cell therapy is a potentially promising option for erectile dysfunction; however, its risk of tumorigenicity is a clinical hurdle and the risk is positively related to the number of injected cells. Our previous study showed that nanotechnology improved adipose-derived stem cell (ADSC) therapy for erectile dysfunction of cavernous nerve injury (CNI) by attracting cells in the corpus cavernosum. These results indicated the possibility of using a reduced dosage of ADSCs for intracavernous injection. In this exploratory study, we used lower dosage (2 × 105 cells) of ADSCs for intracavernous injection (ICI) and the nanotechnology approach. Intracavernous pressure and mean arterial pressure were measured at day 28 to assess erectile function. The low-dose ADSC therapy group showed favorable treatment effects, and nanotechnology further improved these effects. In vivo imaging of ICI cells revealed that the fluorescein signals of NanoShuttle-bound ADSCs (NanoADSCs) were much stronger than those of ADSCs at days 0, 1, and 3. Both immunofluorescence and Western blot analysis showed a significant increase in smooth muscle, endothelium, and nerve tissue in the ADSC group compared to that in the CNI group; further improvement was achieved with assisted nanotechnology. These findings demonstrate that nanotechnology can be used to further improve the effect of small dosage of ADSCs to improve erectile function. Abundant NanoADSCs remain in the corpus cavernosum in vivo for at least 3 days. The mechanism of erectile function improvement may be related to the regeneration of the smooth muscle, endothelium, and nerve tissues.
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影响因子：
3.9
作者：
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通讯作者：
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影响因子：
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DOI：
10.1056/nejmoa1209978
发表时间：
2013-01-31
期刊：
The New England journal of medicine
影响因子：
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作者：
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Penson DF
影响因子：
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作者：
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DOI：
10.1023/a:1024903514335
发表时间：
2003-08-01
影响因子：
3.7
作者：
Kirkpatrick, CJ;Unger, RE;Kamp, G
通讯作者：
Kamp, G