Design and engineering of allosteric communications in proteins.
Design and engineering of allosteric communications in proteins.
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蛋白质变构通讯的设计和工程。
DOI：
10.1016/j.sbi.2022.102334
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发表时间：
2022-04
影响因子：
6.8
通讯作者：
Dokholyan NV
中科院分区：
文献类型：
--
作者：
Chen J;Vishweshwaraiah YL;Dokholyan NV
蛋白质中的变构在调节蛋白质活性、影响基因表达、酶催化和细胞信号转导等生物过程中起着重要作用。当在蛋白质的某一部位检测到的信号通过机械途径传递到功能部位，从而影响其活性时，变构过程就发生了。变构通讯的途径由氨基酸组成，它们以共价键和非共价键形成网络。通过突变这个变构网络中的残基，蛋白质工程师已经成功地建立了新的变构途径，以实现目标蛋白质所需的特性。在这篇综述中，我们重点介绍了变构通信工程的最新和最先进的技术。我们还讨论了蛋白质变构工程需要克服的挑战和未来的发展方向。
Allostery in proteins plays an important role in regulating protein activities and influencing many biological processes such as gene expression, enzyme catalysis, and cell signaling. The process of allostery takes place when a signal detected at a site on a protein is transmitted via a mechanical pathway to a functional site and, thus, influences its activity. The pathway of allosteric communication consists of amino acids that form a network with covalent and non-covalent bonds. By mutating residues in this allosteric network, protein engineers have successfully established novel allosteric pathways to achieve desired properties in the target protein. In this review, we highlight the most recent and state-of-the-art techniques for allosteric communication engineering. We also discuss the challenges that need to be overcome and future directions for engineering protein allostery.
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影响因子：
4.7
作者：
Dagliyan O;Karginov AV;Yagishita S;Gale ME;Wang H;DerMardirossian C;Wells CM;Dokholyan NV;Kasai H;Hahn KM
通讯作者：
Hahn KM
影响因子：
14.8
作者：
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通讯作者：
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作者：
Chen, Zibo;Kibler, Ryan D.;Baker, David
通讯作者：
Baker, David
影响因子：
5.6
作者：
Bingol, Elif Naz;Sercinoglu, Onur;Ozbek, Pemra
通讯作者：
Ozbek, Pemra