Chromatin degradation in differentiating fiber cells of the eye lens.
Chromatin degradation in differentiating fiber cells of the eye lens.
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DOI：
10.1083/jcb.137.1.37
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发表时间：
1997-04-07
影响因子：
7.8
通讯作者：
Mataic, D
中科院分区：
文献类型：
--
作者：
Bassnett, S;Mataic, D
在发育过程中，眼睛的透镜变得透明，部分原因是由于细胞核和其他细胞器通过类似晶状体的机制从中央透镜纤维细胞中消除。使用共聚焦显微镜，我们发现，在无细胞器区（OFZ）的边界，纤维细胞核突然变得不规则的形状，边缘化的染色质。随后，核被膜和下层膜层出现孔洞，核塌陷成致密的球形结构。在OFZ深处检测到含有DNA、组蛋白、核纤层蛋白B2和核膜碎片的核残余物。我们用原位电泳证明，片段化的DNA只存在于毗邻OFZ的细胞中。末端脱氧核苷酸转移酶（TdT）标记的透镜切片的共聚焦显微镜证实，DNA片段化是一个相对较晚的事件在纤维分化，发生后的损失核膜。在正常条件下，在透镜的其他地方未观察到具有3′-OH或3′-PO 4末端的DNA片段，尽管它们可以分别通过DNase I或微球菌核酸酶预处理产生。双标记TdT和抗体对蛋白质二硫键异构酶，ER-居民蛋白，揭示了一个独特的空间和时间之间的差距消失的ER和核膜和DNA降解的发病。因此，纤维细胞染色质解体显着不同于经典的细胞凋亡，在事件的顺序和过程的时间过程。DNA降解仅发生在线粒体、内质网和核膜消失之后，这一事实表明细胞内膜的损伤可能是核破裂的起始事件。
During development, the lens of the eye becomes transparent, in part because of the elimination of nuclei and other organelles from the central lens fiber cells by an apoptotic-like mechanism. Using confocal microscopy we showed that, at the border of the organelle-free zone (OFZ), fiber cell nuclei became suddenly irregular in shape, with marginalized chromatin. Subsequently, holes appeared in the nuclear envelope and underlying laminae, and the nuclei collapsed into condensed, spherical structures. Nuclear remnants, containing DNA, histones, lamin B2, and fragments of nuclear membrane, were detected deep in the OFZ. We used in situ electrophoresis to demonstrate that fragmented DNA was present only in cells bordering the OFZ. Confocal microscopy of terminal deoxynucleotidyl transferase (TdT)–labeled lens slices confirmed that DNA fragmentation was a relatively late event in fiber differentiation, occurring after the loss of the nuclear membrane. DNA fragments with 3′-OH or 3′-PO4 ends were not observed elsewhere in the lens under normal conditions, although they could be produced by pretreatment with DNase I or micrococcal nuclease, respectively. Dual labeling with TdT and an antibody against protein disulfide isomerase, an ER-resident protein, revealed a distinct spatial and temporal gap between the disappearance of ER and nuclear membranes and the onset of DNA degradation. Thus, fiber cell chromatin disassembly differs significantly from classical apoptosis, in both the sequence of events and the time course of the process. The fact that DNA degradation occurs only after the disappearance of mitochondrial, ER, and nuclear membranes suggests that damage to intracellular membranes may be an initiating event in nuclear breakdown.
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DOI：
10.1073/pnas.74.12.5579
发表时间：
1977-01-01
影响因子：
11.1
作者：
APPLEBY, DW;MODAK, SP
通讯作者：
MODAK, SP
影响因子：
3.9
作者：
BARRY, MA;EASTMAN, A
通讯作者：
EASTMAN, A
影响因子：
2.7
作者：
COUNIS, MF;CHAUDUN, E;ALLINQUANT, B
通讯作者：
ALLINQUANT, B
DOI：
10.1016/0922-3371(89)90743-0
发表时间：
1989-07-01
期刊：
CELL DIFFERENTIATION AND DEVELOPMENT
影响因子：
--
作者：
COUNIS, MF;CHAUDUN, E;ALLINQUANT, B
通讯作者：
ALLINQUANT, B
影响因子：
4.7
作者：
BURSCH, W;PAFFE, S;SCHULTEHERMANN, R
通讯作者：
SCHULTEHERMANN, R