Ultrasound localization microscopy to image and assess microvasculature in a rat kidney.
Ultrasound localization microscopy to image and assess microvasculature in a rat kidney.
复制标题
DOI：
10.1038/s41598-017-13676-7
复制
发表时间：
2017-10-20
影响因子：
4.6
通讯作者：
Ferrara KW
中科院分区：
文献类型：
--
作者：
Foiret J;Zhang H;Ilovitsh T;Mahakian L;Tam S;Ferrara KW
超声定位显微镜的最新发展，即在血管系统内检测和跟踪单个微泡(造影剂)，为以低于衍射极限的空间分辨率成像整个器官的血管系统提供了新的机会。在静止组织中，最近的研究证明了微米量级的理论分辨率。在这项工作中，使用专用的高帧速率成像序列在大鼠肾脏中定位了单个微泡。通过假设刚性运动(平移和旋转)来跟踪器官运动，并应用适当的校正。与以前的工作不同，基于相干的非线性相位反转处理被用来抑制组织回波，同时保持来自非常缓慢运动的微泡的回波。通过跟踪微泡来估计小血管中的血流速度，展示了这项技术改善血管特征的潜力。以前对大约20微米血管中的微气泡的光学研究表明，膨胀受到限制，这表明在这些区域很难检测到微气泡回声。因此，我们利用单个MB的回声作为与此类血管相关的慢流的微观传感器，并证明了在流速低于2 mm/S的血管中，从单个微泡中可以检测到高度相关的宽带回声。
The recent development of ultrasound localization microscopy, where individual microbubbles (contrast agents) are detected and tracked within the vasculature, provides new opportunities for imaging the vasculature of entire organs with a spatial resolution below the diffraction limit. In stationary tissue, recent studies have demonstrated a theoretical resolution on the order of microns. In this work, single microbubbles were localized in vivo in a rat kidney using a dedicated high frame rate imaging sequence. Organ motion was tracked by assuming rigid motion (translation and rotation) and appropriate correction was applied. In contrast to previous work, coherence-based non-linear phase inversion processing was used to reject tissue echoes while maintaining echoes from very slowly moving microbubbles. Blood velocity in the small vessels was estimated by tracking microbubbles, demonstrating the potential of this technique to improve vascular characterization. Previous optical studies of microbubbles in vessels of approximately 20 microns have shown that expansion is constrained, suggesting that microbubble echoes would be difficult to detect in such regions. We therefore utilized the echoes from individual MBs as microscopic sensors of slow flow associated with such vessels and demonstrate that highly correlated, wideband echoes are detected from individual microbubbles in vessels with flow rates below 2 mm/s.
登录
查看更多内容
影响因子：
4.2
作者：
Hingot, Vincent;Errico, Claudia;Couture, Olivier
通讯作者：
Couture, Olivier
影响因子：
10.6
作者：
Demene, Charlie;Deffieux, Thomas;Tanter, Mickael
通讯作者：
Tanter, Mickael
影响因子：
37.8
作者：
Ellegala, DB;Poi, HL;Lindner, JR
通讯作者：
Lindner, JR
DOI：
10.1109/tuffc.2012.2508
发表时间：
2012-12-01
影响因子：
3.6
作者：
Couture, Olivier;Fink, Mathias;Tanter, Mickael
通讯作者：
Tanter, Mickael
影响因子：
2.4
作者：
Caskey, Charles F.;Stieger, Susanne M.;Ferrara, Katherine W.
通讯作者：
Ferrara, Katherine W.