Mistimed restricted feeding disrupts circadian rhythms of male mating behavior and female preovulatory LH surges in mice.
Mistimed restricted feeding disrupts circadian rhythms of male mating behavior and female preovulatory LH surges in mice.
复制标题
DOI：
10.1016/j.yhbeh.2022.105242
复制
发表时间：
2022-09
影响因子：
3.5
通讯作者：
Butler, Matthew P.
中科院分区：
文献类型：
--
作者：
Kukino, Ayaka;Walbeek, Thijs J.;Sun, Lori J.;Watt, Alexander T.;Park, Jin Ho;Kauffman, Alexander S.;Butler, Matthew P.
关键词：
在啮齿类动物中，在非典型的昼夜节律时间进食，例如在生物休息阶段进食通常是最小的，会降低生育能力。先前的研究结果表明，这种生育能力受损至少部分是由于交配成功率降低。然而，这种生殖抑制的生理和行为机制尚不清楚。在本研究中，我们测试了这一假设，即不合时宜的喂养引起的不孕症是由于在正常的昼夜节律的交配行为和/或排卵前促黄体生成激素（LH）激增（雌激素正反馈）的产生中断。在第一个实验中，雄性+雌性小鼠对，适应食物限制在光照（不定时喂养）或黑暗（对照喂养）阶段，在96小时内对安装频率和射精进行评分。雄性的安装行为和射精分布更广泛的一天在光喂养的小鼠比黑暗喂养的控制和更少的光喂养的男性射精。在第二个实验中，LH峰的时间，一个由雌二醇（E2）和SCN驱动的充分表征的昼夜节律事件，从卵巢切除和E2致敏的雌性小鼠的一系列血液样本中进行了分析，这些小鼠是光，暗，或自由进食。与预期一致，在黑暗喂养和自由进食对照雌性动物中，LH浓度在熄灯后2小时达到峰值，但在光照喂养雌性动物中未达到峰值。相反，正常聚集的LH峰分布广泛，在光饲组小鼠间变异性较高。这些数据表明，不适时的喂养扰乱了排卵和交配行为背后的神经过程的时间控制，导致生育力低下。
In rodents, eating at atypical circadian times, such as during the biological rest phase when feeding is normally minimal, reduces fertility. Prior findings suggest this fertility impairment is due, at least in part, to reduced mating success. However, the physiological and behavioral mechanisms underlying this reproductive suppression are not known. In the present study, we tested the hypothesis that mistimed feeding-induced infertility is due to a disruption in the normal circadian timing of mating behavior and/or the generation of pre-ovulatory luteinizing hormone (LH) surges (estrogen positive feedback). In the first experiment, male+female mouse pairs, acclimated to be food restricted to either the light (mistimed feeding) or dark (control feeding) phase, were scored for mounting frequency and ejaculations over 96 hours. Male mounting behavior and ejaculations were distributed much more widely across the day in light-fed mice than in dark-fed controls and fewer light-fed males ejaculated. In the second experiment, the timing of the LH surge, a well characterized circadian event driven by estradiol (E2) and the SCN, was analyzed from serial blood samples taken from ovariectomized and E2-primed female mice that were light-, dark-, or ad-lib-fed. LH concentrations peaked 2h after lights-off in both dark-fed and ad-lib control females, as expected, but not in light-fed females. Instead, the normally clustered LH surges were distributed widely with high inter-mouse variability in the light-fed group. These data indicate that mistimed feeding disrupts the temporal control of the neural processes underlying both ovulation and mating behavior, contributing to subfertility.
登录
查看更多内容
影响因子：
4.8
作者：
EVERETT, JW;SAWYER, CH
通讯作者：
SAWYER, CH
影响因子：
29
作者：
Hatori M;Vollmers C;Zarrinpar A;DiTacchio L;Bushong EA;Gill S;Leblanc M;Chaix A;Joens M;Fitzpatrick JA;Ellisman MH;Panda S
通讯作者：
Panda S
DOI：
10.1007/s003590050017
发表时间：
2000-02-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY A-SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
影响因子：
--
作者：
DeCoursey, PJ;Walker, JK;Smith, SA
通讯作者：
Smith, SA
影响因子：
3.6
作者：
Dror, Tal;Franks, Jennifer;Kauffman, Alexander S.
通讯作者：
Kauffman, Alexander S.
影响因子：
3.5
作者：
Alvarez, J. D.;Hansen, Amanda;Sehgal, Amita
通讯作者：
Sehgal, Amita