Comparative analysis of P2X1, P2X2, P2X3, and P2X4 receptor subunits in rat nodose ganglion neurons.
Comparative analysis of P2X1, P2X2, P2X3, and P2X4 receptor subunits in rat nodose ganglion neurons.
复制标题
大鼠结节神经元 P2X1、P2X2、P2X3 和 P2X4 受体亚基的比较分析
DOI：
10.1371/journal.pone.0096699
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Pei L
中科院分区：
文献类型：
--
作者：
Wang L;Feng D;Yan H;Wang Z;Pei L
结状神经节（NG）神经元是内脏初级感觉神经元。内脏感觉的传递和调节主要由P2 X嘌呤受体（P2 X receptor）介导。虽然以前已经研究了NG中不同P2 X受体亚基的特征，但尚未进行全面的分析。在这项研究中，我们使用免疫组织化学，免疫细胞化学和全细胞膜片钳技术比较P2 X1，P2 X2，P2 X3和P2 X4受体亚单位在成年大鼠NG神经元的表达和功能。针对四个P2 X亚基的多克隆抗体标记NG神经元的不同亚群。P2 X1和P2 X3主要表达于中小型NG神经元，而P2 X2和P2 X4主要位于中型和大型NG神经元。NG神经元中P2 X3阳性率超过36%，高于其他三种P2 X亚基。此外，从表达不同P2 X亚基的神经元记录到不同类型的电流。快型ATP电流记录自含有P2 X1 -4亚基的神经元，中间型电流记录自含有P2 X1、P2 X3和P2 X4亚基的神经元，慢型电流记录自表达P2 X1 -3和/或P2 X4亚基的神经元，而极慢型电流记录自含有P2 X2和P2 X3亚基的神经元。这些比较结果提供了NG神经元中不同亚基的解剖学验证，并为各种功能性NG群体对ATP具有不同反应的想法提供了直接支持，这可能部分是由于不同P2 X亚基的不同表达谱。
Nodose ganglion (NG) neurons are visceral primary sensory neurons. The transmission and regulation of visceral sensation is mediated mainly by the P2X purinoceptor (P2X receptor). Although the characteristics of different P2X receptor subunits in the NG have been studied previously, comprehensive analyses have not been performed. In this study, we used immunohistochemistry, immunocytochemistry, and whole cell patch clamp techniques to compare the expression and function of P2X1, P2X2, P2X3, and P2X4 receptor subunits in adult rat NG neurons. Polyclonal antibodies against the four P2X subunits labeled different subpopulations of NG neurons. P2X1 and P2X3 were expressed mainly in small-to-medium sized NG neurons, whereas P2X2 and P2X4 were located mostly in medium- and larger-sized NG neurons. Over 36% of NG neurons were P2X3 positive, which was higher than the other three P2X subunits. In addition, different types of currents were recorded from neurons expressing different P2X subunits. The fast type of ATP current was recorded from neurons containing P2X1–4 subunits, the intermediate type of current was recorded from neurons containing the P2X1, P2X3, and P2X4 subunits, the slow type was recorded from neurons expressing P2X1–3, and/or P2X4 subunits, whereas the very slow type was recorded from neurons containing the P2X2 and P2X3 subunits. These comparative results provide an anatomical verification of the different subunits in NG neurons, and offer direct support for the idea that various functional NG populations have distinct responses to ATP, which might be in part due to the different expression profiles of diverse P2X subunits.
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DOI：
10.1124/jpet.300.2.673
发表时间：
2002-02-01
影响因子：
3.5
作者：
Brown, SG;Townsend-Nicholson, A;King, BF
通讯作者：
King, BF
DOI：
10.1111/j.1749-6632.1999.tb11351.x
发表时间：
1999-01-01
期刊：
MOLECULAR AND FUNCTIONAL DIVERSITY OF ION CHANNELS AND RECEPTORS
影响因子：
--
作者：
MacKenzie, AB;Surprenant, A;North, RA
通讯作者：
North, RA
影响因子：
4.8
作者：
Boué-Grabot, É;Archambault, V;Séguéla, P
通讯作者：
Séguéla, P
影响因子：
0.5
作者：
Bobryshev, A. Yu.;Petrushenko, E. A.;Krishtal, O. A.
通讯作者：
Krishtal, O. A.
影响因子：
7.3
作者：
Dunn, PM;Liu, M;Burnstock, G
通讯作者：
Burnstock, G