A Putative Role of Vasopressin/Oxytocin-Type Neuropeptide in Osmoregulation and Feeding Inhibition of Apostichopus japonicus.
A Putative Role of Vasopressin/Oxytocin-Type Neuropeptide in Osmoregulation and Feeding Inhibition of Apostichopus japonicus.
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DOI：
10.3390/ijms241814358
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发表时间：
2023-09-20
影响因子：
5.6
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Cong X;Liu H;Zheng Y;Chen M
加压素/催产素（VP/OT）型神经肽是一种古老的神经垂体素相关神经肽，在原口类和后口类脊椎动物中参与多种生理过程。然而，VP/OT型神经肽在后口无脊椎动物尤其是棘皮动物中的功能却知之甚少。在此，我们首次报道了在亚洲广泛养殖的具有很高营养和药用价值的重要经济物种仿刺参中VP/OT型神经肽信号传导。全催产素及其前体的分子特征分析揭示了VP/OT家族的高度保守性。全催产素的候选受体AjHOR被证实能够通过cAMP-PKA和可能的Ca ~（2+）-PKC途径激活信号传导，并进一步激活下游的ERK 1/2级联。催产素前体表达谱显示，它们主要集中在口周神经环。此外，体外药理学实验表明，holotocin引起的收缩反应，从A。- 是的体内功能研究表明，短期注射催产素可导致体膨胀，长期注射催产素可导致体质量下降，提示催产素可能在摄食调节和与催产素-CCK的摄食共抑制中发挥作用。我们的研究结果提供了一个全面的描述AjHOR-holotocin信号，揭示了古老的角色，holotocin的调节和摄食抑制通过控制肌肉收缩。
Vasopressin/oxytocin (VP/OT)-type neuropeptide is an ancient neurophysin-associated neuropeptide and has been intensively studied to be involved in multiple physiological processes in protostomian and deuterostome vertebrates. However, little is known about the functions of VP/OT-type neuropeptide in deuterostome invertebrates especially in echinoderms. Here, we firstly report VP/OT-type neuropeptide signaling in an important economic species, Apostichopus japonicus, which is widely cultured in Asia, with high nutritional and medicinal values. Molecular characterization analysis of holotocin and its precursor revealed the highly conserved features of VP/OT family. The candidate receptor for holotocin (AjHOR) was confirmed to be able to activate the signaling via cAMP-PKA and possible Ca2+-PKC pathway, and further activated the downstream ERK1/2 cascade. Holotocin precursor expression profile showed that they were mainly concentrated in circumoral nerve ring. Furthermore, in vitro pharmacological experiments demonstrated that holotocin caused contractile responses in preparations from A. japonicus. And in vivo functional studies indicated that short-term injection of holotocin resulted in body bloat and long-term injection resulted in reduced body mass, suggesting potential roles of holotocin in osmoregulation and feeding co-inhibition with holotocin–CCK. Our findings provided a comprehensive description of AjHOR–holotocin signaling, revealed ancient roles of holotocin in osmoregulation and feeding inhibition by controlling muscle contractions.
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影响因子：
3.8
作者：
Aikins, Michael J.;Schooley, David A.;Park, Yoonseong
通讯作者：
Park, Yoonseong
影响因子：
4.3
作者：
Gaillard AL;Tay BH;Pérez Sirkin DI;Lafont AG;De Flori C;Vissio PG;Mazan S;Dufour S;Venkatesh B;Tostivint H
通讯作者：
Tostivint H
急性盐度应激对海参apostichopus japonicus中离子稳态，Na（+）/K（+） - ATPase和组织学结构的影响。
影响因子：
--
作者：
Geng C;Tian Y;Shang Y;Wang L;Jiang Y;Chang Y
通讯作者：
Chang Y
DOI：
10.1126/science.1226201
发表时间：
2012-10-26
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Garrison JL;Macosko EZ;Bernstein S;Pokala N;Albrecht DR;Bargmann CI
通讯作者：
Bargmann CI
影响因子：
2.8
作者：
Elphick, Maurice R.;Rowe, Matthew L.
通讯作者：
Rowe, Matthew L.