Effect of acute salinity stress on ion homeostasis, Na(+)/K(+)-ATPase and histological structure in sea cucumber Apostichopus japonicus.
Effect of acute salinity stress on ion homeostasis, Na(+)/K(+)-ATPase and histological structure in sea cucumber Apostichopus japonicus.
复制标题
急性盐度应激对海参apostichopus japonicus中离子稳态，Na（+）/K（+） - ATPase和组织学结构的影响。
DOI：
10.1186/s40064-016-3620-4
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chang Y
中科院分区：
文献类型：
--
作者：
Geng C;Tian Y;Shang Y;Wang L;Jiang Y;Chang Y
海参（Apostichopus japonicus）是一种濒临灭绝的动物群，暴露于盐度等各种环境条件下，迫切需要进行研究来评估其影响以指导水产养殖工作。通过测定急性盐度胁迫对呼吸树体腔液渗透压、离子浓度、Na+/K+-ATP酶活性及组织学变化的影响，评价海参的耐盐能力。在体腔液和周围环境盐度之间观察到渗透压的显着相关性。在体腔液中，观察到 Na+ 浓度在盐度 18 psu 期间波动，并在 6 小时出现拐点。呼吸树Na+/K+-ATP酶活性呈现“U型”波动变化，且变化趋势与Na+浓度相反。离子 (K+、Cl−) 浓度下降，并在盐度 40 psu 和盐度 18 psu 时表现出相同的趋势。体腔细胞总数和体腔液Na+/K+-ATP酶活性的吞噬作用在不同盐度胁迫下呈现波动变化。组织学变化揭示了盐浓度降低与组织完整性之间的负相关。在低盐度环境（18、23 和 27 psu）下，肠道、肌肉和管足中观察到明显的组织损伤。暴露于 18 和 23 psu 的刺参肠道结缔组织受损，部分与粘膜上皮分离。显着的变化发生在管脚上，在低盐度（18 psu）下表现为结缔组织肿胀和纵向肌肉断裂。管脚的形态变化表现为高盐度（40 psu）下结缔组织的收缩。在盐度处理组（18 和 23 psu）中，呼吸树中的滴虫数量减少甚至消失。结果推断海参存在渗透一致和离子调节，这有助于了解刺参在急性盐胁迫下的盐度调节机制。
Sea cucumbers (Apostichopus japonicus) are an imperiled fauna exposed to a variety of environmental condition such as salinity and studies are urgently needed to assess their effects to guide aquaculture efforts. The effects of acute salinity stress on coelomic fluid osmotic pressure, ion concentrations, the activity of Na+/K+-ATPase in respiratory trees and the histological variations were measured to evaluate the salinity tolerance of sea cucumbers. Significant correlations in osmotic pressure were observed between coelomic fluid and ambient environmental salinity. In coelomic fluid, Na+ concentration was observed fluctuated during salinity 18 psu and the inflection point presented at the 6 h. The Na+/K+-ATPase activity in respiratory trees indicated the “U-shaped” fluctuant change and the change trend was opposite with the Na+ concentration. The ions (K+, Cl−) concentration decreased and showed the same tendency at salinity 40 psu with salinity 18 psu. The total coelomocytes counts and phagocytosis of coelomic fluid Na+/K+-ATPase activity indicated fluctuating changes under different salinity stress. Histological variation revealed a negative relation between decreasing salt concentration and tissue integrity. Tissue damages were significantly observed in intestines, muscles and tube feet under low salinity environment (18, 23 and 27 psu). The connective tissue in intestines of A. japonicus exposed to 18 and 23 psu damaged and partly separated from the mucosal epithelium. The significant variations occurred in tube feet, which presented the swelling in connective tissue and a fracture in longitudinal muscles under low salinity (18 psu). The morphological change of tube feet showed the shrinkage of connective tissue under high salinity (40 psu). The amount of infusoria in the respiratory trees decreased or even disappeared in salinity treatment groups (18 and 23 psu). The results inferred that osmoconformity and ionoregulation were seen in sea cucumbers, which contributed to understand the salinity regulatory mechanisms of A. japonicus under acute salinity stress.
登录
查看更多内容
影响因子：
4.5
作者：
Bai, Yucen;Zhang, Libin;Yang, Hongsheng
通讯作者：
Yang, Hongsheng
DOI：
10.1016/j.cbpa.2006.02.003
发表时间：
2006-05-01
影响因子：
2.3
作者：
Chung, Kuei-Fang;Lin, Hui-Chen
通讯作者：
Lin, Hui-Chen
影响因子：
3
作者：
Hennebert, Elise;Viville, Pascal;Flammang, Patrick
通讯作者：
Flammang, Patrick
影响因子：
4.5
作者：
Fielder, D. Stewart;Allan, Geoff L.;Pankhurst, Patricia M.
通讯作者：
Pankhurst, Patricia M.
影响因子：
4.5
作者：
Do Thi Thanh Huong;Jasmani, Safiah;Wilder, Marcy
通讯作者：
Wilder, Marcy