A method for assessing mitochondrial bioenergetics in whole white adipose tissues.
A method for assessing mitochondrial bioenergetics in whole white adipose tissues.
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一种评估整个白色脂肪组织中线粒体生物能学的方法。
DOI：
10.1016/j.redox.2014.04.005
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发表时间：
2014
期刊：
影响因子：
11.4
通讯作者：
Ballinger SW
中科院分区：
文献类型：
--
作者：
Dunham-Snary KJ;Sandel MW;Westbrook DG;Ballinger SW
肥胖是许多代谢性疾病的主要危险因素，包括代谢综合征、2型糖尿病、心血管疾病和癌症。尽管传统上认为白色脂肪组织是一种储存器官，但白色脂肪组织生物学领域正在扩大，包括将该组织视为内分泌器官和整体代谢的主要贡献者。鉴于其在能量产生中的作用，线粒体一直是代谢功能障碍研究的焦点，并且细胞器与白色脂肪组织功能之间可能存在联系。在此，我们提出了一种评估整个白色脂肪组织线粒体生物能量的新方法。这种方法需要最小的组织操作，并且不需要细胞分离和培养。此外，该方法克服了处理转化和/或分离原代细胞的一些限制，并允许更方便地获得结果。除了新的方法，我们提出了一个全面的统计分析的生物能量数据，以及异常值分析的指导方针。三分之一的美国人患有肥胖症和代谢性疾病；发病率正在上升。WAT作为内分泌器官和代谢的主要贡献者正在崛起。线粒体与白色脂肪组织之间的关系有待进一步探讨。目前的生物能量学方法仅限于转化或培养原代细胞。提出了一种新的组织制备、数据采集和分析方法。
Obesity is a primary risk factor for numerous metabolic diseases including metabolic syndrome, type II diabetes (T2DM), cardiovascular disease and cancer. Although classically viewed as a storage organ, the field of white adipose tissue biology is expanding to include the consideration of the tissue as an endocrine organ and major contributor to overall metabolism. Given its role in energy production, the mitochondrion has long been a focus of study in metabolic dysfunction and a link between the organelle and white adipose tissue function is likely. Herein, we present a novel method for assessing mitochondrial bioenergetics from whole white adipose tissue. This method requires minimal manipulation of tissue, and eliminates the need for cell isolation and culture. Additionally, this method overcomes some of the limitations to working with transformed and/or isolated primary cells and allows for results to be obtained more expediently. In addition to the novel method, we present a comprehensive statistical analysis of bioenergetic data as well as guidelines for outlier analysis. Obesity and metabolic disease affect 1/3 of the US population; incidence is rising. WAT is emerging as an endocrine organ and major contributor to metabolism. The relationship between mitochondria and white adipose tissue needs to be explored. Current bioenergetics methods are limited to transformed or cultured primary cells. A novel method for tissue preparation, data acquisition and analysis is presented.
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影响因子：
7.4
作者：
Gerencser, Akos A.;Neilson, Andy;Choi, Sung W.;Edman, Ursula;Yadava, Nagendra;Oh, Richard J.;Ferrick, David A.;Nicholls, David G.;Brand, Martin D.
通讯作者：
Brand, Martin D.
影响因子：
20.1
作者：
Sansbury BE;Cummins TD;Tang Y;Hellmann J;Holden CR;Harbeson MA;Chen Y;Patel RP;Spite M;Bhatnagar A;Hill BG
通讯作者：
Hill BG
影响因子：
7.4
作者：
Dranka, Brian P.;Hill, Bradford G.;Darley-Usmar, Victor M.
通讯作者：
Darley-Usmar, Victor M.
影响因子：
8.2
作者：
Choo, HJ;Kim, JH;Ko, YG
通讯作者：
Ko, YG
影响因子：
6.9
作者：
Ahima, Rexford S.
通讯作者：
Ahima, Rexford S.