Structural and functional characterization of glycosylation in an immunoglobulin G1 to Cryptococcus neoformans glucuronoxylomannan.
Structural and functional characterization of glycosylation in an immunoglobulin G1 to Cryptococcus neoformans glucuronoxylomannan.
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新型隐球菌葡糖醛酸木甘露聚糖免疫球蛋白 G1 糖基化的结构和功能表征。
DOI：
10.1016/j.molimm.2005.05.013
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发表时间：
2006
影响因子：
3.6
通讯作者：
Casadevall,Arturo
中科院分区：
文献类型：
--
作者：
Wang,Fang;Nakouzi,Antonio;Alvarez,Mauricio;Zaragoza,Oscar;Angeletti,RuthHogue;Casadevall,Arturo
通过LC/MS分析新型隐球菌鼠IgG 1单克隆抗体（mAb）的N-连接寡糖，发现Asn-293处有5种不同的核心岩藻糖基化双触角复合型寡糖，主要种类为糖基组成为Hex 4 HexNAc 4Fuc的单半乳糖基化寡糖（占总聚糖池的39%）。从核酸测序预测的一级序列不同于通过质谱法在位置33（从天冬氨酸到ASP）测量的一级序列，这一发现可能代表由自发ASP脱氨基引起的翻译后修饰。对来自三个杂交瘤克隆的mAb 18 B7的分析显示了相同的异质N-聚糖模式，表明低聚糖结构的多样性源自个体细胞。天然和去糖基化mAb 18 B7与隐球菌Ag的结合相当，但去糖基化18 B7具有较短的血清半衰期，并且不激活补体（C）。去糖基化mAb 18 B7对C.新生儿与鼠巨噬细胞通过一种机制，涉及C-非依赖性摄取通过C受体。被动给予去糖基化mAb 18 B7在致死性感染小鼠中介导的保护效力与天然mAb相当。结果表明，N-聚糖结构对免疫球蛋白功能的贡献取决于Ag-Ab系统。
Analysis of the N-linked oligosaccharides of the murine IgG1 monoclonal antibody (mAb) to Cryptococcus neoformans by LC/MS revealed five different core fucosylated, biantennary complex-type oligosaccharides at Asn-293, with the major species being a mono-galactosylated oligosaccharide with the glycosyl composition of Hex4HexNAc4Fuc (39% of the total glycan pool). The primary sequence predicted from nucleic acid sequencing differed from that measured by mass spectrometry at position 33 (ASN to ASP), a finding that may represent post-translational modification caused by spontaneous ASP deamination. Analysis of mAb 18B7 from three hybridoma clones revealed the same heterogenous N-glycan pattern, indicating that diversity in oligosaccharide structures originated from individual cells. The binding of native and de-glycosylated mAb 18B7 to cryptococcal Ag was comparable but the de-glycosylated 18B7 had shorter serum half-life and did not activate complement (C). De-glycosylated mAb 18B7 was opsonic for C. neoformans with murine macrophages through a mechanism that involved C-independent ingestion through the C receptor. Passive administration of de-glycosylated mAb 18B7 mediated comparable protective efficacy to the native mAb in mice with lethal infection. The results imply that the contribution of N-glycan structure to immunoglobulin function varies depending on the Ag–Ab system.
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影响因子：
17.3
作者：
A. Wright;S. Morrison
通讯作者：
A. Wright;S. Morrison
小鼠免疫球蛋白 G2b 铰链区的 O-糖基化。
影响因子：
4.8
作者：
Hahyung Kim;Y. Yamaguchi;K. Masuda;Chigusa Matsunaga;Kazuo Yamamoto;T. Irimura;N. Takahashi;K. Kato;Y. Arata
通讯作者：
Y. Arata
蛋白质抗生素新制癌菌素在弱酸性 pH 条件下自发脱酰胺。
DOI：
--
发表时间：
1977
期刊：
Journal of Biochemistry (Tokyo)
影响因子：
--
作者：
H. Maeda;K. Kuromizu
通讯作者：
K. Kuromizu
影响因子：
4.3
作者：
Wright, A;Sato, Y;Morrison, SL
通讯作者：
Morrison, SL
网状细胞肉瘤：抗体依赖性细胞介导的免疫中的效应细胞。
影响因子：
4.4
作者：
P. Ralph;J. Prichard;M. Cohn
通讯作者：
M. Cohn