Separation of developmental compartments by a cell type with reduced junctional permeability-猫眼课题宝

通过连接通透性降低的细胞类型分隔发育区室

DOI：

发表时间：

1984

期刊：

Nature

影响因子：

64.8

通讯作者：

中科院分区：

综合性期刊1区

文献类型：

作者：

Michael G. Blennerhassett;Stanley Caveney

最近的两份报告 1,2 表明，位于发育中的昆虫上皮细胞的区室边界处的细胞连接膜虽然可渗透无机离子 3,4，但阻止了荧光示踪剂荧光黄 (LY) 的细胞间转移。然而，隔室内的细胞通过可渗透无机离子和有机示踪剂的连接点连接1-5。这些发现意味着连接通讯在组织发育中很重要，并且空间选择性可能允许附近区室的发育自主性。观察到 LY 偶尔会穿过马利筋虫 Oncopeltus fasciatus1 的节段边界，这表明节段边界连接处的渗透性可能是局部控制的。我们在此报告，这种昆虫的区室边界处的连接通道的选择性是可变的，并且受到离散的边界细胞群体的控制。相对于隔室内的示踪剂穿过隔室边界的速率，这些细胞降低的程度受到放置组织的培养基的影响。

Two recent reports1,2 have suggested that the junctional membrane of the cells located at compartment boundaries in developing insect epithelia, although permeable to inorganic ions3,4, prevents the cell-to-cell transfer of the fluorescent tracer Lucifer yellow (LY). However, cells within a compartment are linked by junctions permeable to both inorganic ions and organic tracers1–5. These findings imply that junctional communication is important in tissue development and that spatial selectivity may allow developmental autonomy in nearby compartments. The observation that LY was occasionally seen to pass across the segment border of the milkweed bug Oncopeltus fasciatus1 suggested to us that the permeability of the junctions of the segment border may be locally controlled. We report here that the selectivity in the junctional channels at compartment boundaries in this insect is variable and under the control of a discrete population of border cells. The extent to which these cells reduce the rate of tracer passage across the compartment boundary relative to that within the compartment is influenced by the culture medium in which the tissue is placed.

Gap junctional communication compartments in the Drosophila wing disk.