DDR2, a discoidin domain receptor, is a marker of periosteal osteoblast and osteoblast progenitors.
DDR2, a discoidin domain receptor, is a marker of periosteal osteoblast and osteoblast progenitors.
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DDR2 是一种盘状结构域受体，是骨膜成骨细胞和成骨细胞祖细胞的标记物
DOI：
10.1007/s00774-020-01108-y
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发表时间：
2020-09
影响因子：
3.3
通讯作者：
Duan J
中科院分区：
文献类型：
--
作者：
Yang H;Sun L;Cai W;Gu J;Xu D;Deb A;Duan J
骨膜具有双层结构，包围着皮质骨。外层富含结缔组织和成纤维细胞，而与骨皮质表面接触的内层主要由成骨细胞和成骨细胞祖细胞组成。鉴定骨膜的双层结构的细胞特异性表面标志物是重要的，为目的的组织regeneration.Materials和methodsWe调查的discoidin域酪氨酸激酶受体DDR2，成纤维细胞特异性蛋白-1（FSP-1）和碱性磷酸酶（ALP）在皮质骨骨膜免疫组化的表达。DDR2和FSP-1表达细胞之间的成骨分化进行了比较，流式分选从periosteum.ResultsWe表明，DDR2主要标记的成骨细胞居住在内层的骨膜和皮尔逊的共定位系数表明与ALP的表达显着相关。从骨膜分离的表达DDR2的成骨细胞的矿化被显著诱导。相反，FSP-1主要标记骨膜成纤维细胞的外层，Pearson共定位系数表明FSP-1与DDR2和ALP的表达相关性很差。结论DDR2是一种新的细胞表面标记物，可用于鉴定和分离骨膜内的成骨细胞和成骨祖细胞，可用于肌肉骨骼再生治疗。
IntroductionThe periosteum has a bilayered structure that surrounds cortical bone. The outer layer is rich in connective tissue and fibroblasts, while the inner layer in contact with the cortical surface of the bone predominantly consists of osteoblasts and osteoblast progenitors. The identification of cell-specific surface markers of the bilayered structure of the periosteum is important for the purpose of tissue regeneration.Materials and methodsWe investigated the expression of the discoidin domain tyrosine kinase receptor DDR2, fibroblast specific protein-1 (FSP-1) and alkaline phosphatase (ALP) in the periosteum of cortical bone by immunohistochemistry. Osteogenic differentiation was compared between DDR2- and FSP-1-expressing cells flow-sorted from the periosteum.ResultsWe showed that DDR2 predominantly labeled osteogenic cells residing in the inner layer of the periosteum and that Pearson’s coefficient of colocalization indicated a significant correlation with the expression of ALP. The mineralization of DDR2-expressing osteogenic cells isolated from the periosteum was significantly induced. In contrast, FSP-1 predominantly labeled the outer layer of periosteal fibroblasts, and Pearson’s coefficient of colocalization indicated that FSP-1 was poorly correlated with the expression of DDR2 and ALP. FSP-1-expressing periosteal fibroblasts did not exhibit osteogenic differentiation for the induction of bone mineralization.ConclusionDDR2 is a novel potential cell surface marker for identifying and isolating osteoblasts and osteoblast progenitors within the periosteum that can be used for musculoskeletal regenerative therapies.
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影响因子：
3.5
作者：
Park, J;Gelse, K;Schneider, H
通讯作者：
Schneider, H
影响因子：
9.8
作者：
Bargal, Ruth;Cormier-Daire, Valerie;Raas-Rothschild, Annick
通讯作者：
Raas-Rothschild, Annick
影响因子：
4.1
作者：
Arnsdorf, Emily J.;Jones, Luis M.;Jacobs, Christopher R.
通讯作者：
Jacobs, Christopher R.
影响因子：
1.7
作者：
Ng, AMH;Bin Saim, A;Idrus, RBH
通讯作者：
Idrus, RBH
DOI：
10.1083/jcb.130.2.393
发表时间：
1995-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Strutz F;Okada H;Lo CW;Danoff T;Carone RL;Tomaszewski JE;Neilson EG
通讯作者：
Neilson EG