Antibodies to the core proteins of Nairobi sheep disease virus/Ganjam virus reveal details of the distribution of the proteins in infected cells and tissues.
Antibodies to the core proteins of Nairobi sheep disease virus/Ganjam virus reveal details of the distribution of the proteins in infected cells and tissues.
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DOI：
10.1371/journal.pone.0124966
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Baron MD
中科院分区：
文献类型：
--
作者：
Lasecka L;Bin-Tarif A;Bridgen A;Juleff N;Waters RA;Baron MD
内罗毕羊病病毒（NSDV；在印度也称为 Ganjam 病毒）是内罗病毒属的布尼亚病毒。它会导致绵羊和山羊出血性胃肠炎，死亡率高达 90%。该病毒与人类病原体克里米亚-刚果出血热病毒（CCHFV）密切相关。目前对 NSDV 的生物学知之甚少。我们已经生成了针对病毒核衣壳蛋白 (N) 和聚合酶 (L) 的特异性抗体，并使用这些抗体来表征受感染细胞中的 NSDV 并研究其在自然宿主感染过程中的分布。由于其较大的尺寸和木瓜蛋白酶样蛋白酶（OTU 样结构域）的存在，有人认为内罗病毒的 L 蛋白会经历自蛋白水解裂解成聚合酶和一种或多种辅助蛋白。识别 NSDV L 蛋白 N 端或 C 端的特异性抗体没有显示出 NSDV 感染细胞中 L 蛋白裂解的证据。使用特异性抗 N 和抗 L 抗体，发现这些病毒蛋白并未完全共定位于受感染的细胞中； N蛋白在感染早期聚集在高尔基体附近，而L蛋白分布在整个细胞质中，进一步支持了L蛋白的多功能性质。这些抗体还使我们能够获得有关体内病毒靶向的器官和细胞类型的信息。我们可以在从实验接种动物死后收集的各种组织制备的冰冻切片中检测 NSDV；该病毒存在于小肠和大肠的粘膜内层、肺部和肠系膜淋巴结 (MLN) 中，NSDV 似乎在这些淋巴结中以单核细胞和/或巨噬细胞为目标。
Nairobi sheep disease virus (NSDV; also called Ganjam virus in India) is a bunyavirus of the genus Nairovirus. It causes a haemorrhagic gastroenteritis in sheep and goats with mortality up to 90%. The virus is closely related to the human pathogen Crimean-Congo haemorrhagic fever virus (CCHFV). Little is currently known about the biology of NSDV. We have generated specific antibodies against the virus nucleocapsid protein (N) and polymerase (L) and used these to characterise NSDV in infected cells and to study its distribution during infection in a natural host. Due to its large size and the presence of a papain-like protease (the OTU-like domain) it has been suggested that the L protein of nairoviruses undergoes an autoproteolytic cleavage into polymerase and one or more accessory proteins. Specific antibodies which recognise either the N-terminus or the C-terminus of the NSDV L protein showed no evidence of L protein cleavage in NSDV-infected cells. Using the specific anti-N and anti-L antibodies, it was found that these viral proteins do not fully colocalise in infected cells; the N protein accumulated near the Golgi at early stages of infection while the L protein was distributed throughout the cytoplasm, further supporting the multifunctional nature of the L protein. These antibodies also allowed us to gain information about the organs and cell types targeted by the virus in vivo. We could detect NSDV in cryosections prepared from various tissues collected post-mortem from experimentally inoculated animals; the virus was found in the mucosal lining of the small and large intestine, in the lungs, and in mesenteric lymph nodes (MLN), where NSDV appeared to target monocytes and/or macrophages.
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DOI：
10.4269/ajtmh.1980.29.941
发表时间：
1980-01-01
影响因子：
3.3
作者：
BURNEY, MI;GHAFOOR, A;CASALS, J
通讯作者：
CASALS, J
DOI：
10.1016/s0006-291x(03)00881-7
发表时间：
2003-06-13
影响因子：
3.1
作者：
Choi, J;Chang, JS;Han, YS
通讯作者：
Han, YS
影响因子：
5.4
作者：
Bosch, I;Xhaja, K;Rothman, AL
通讯作者：
Rothman, AL
影响因子：
3.7
作者：
Bridgen, A;Dalrymple, DA;Elliott, RM
通讯作者：
Elliott, RM
影响因子：
7.7
作者：
Balakirev, MY;Tcherniuk, SO;Chroboczek, J
通讯作者：
Chroboczek, J