Anti-Inflammatory Effect of Turbo cornutus Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study.
Anti-Inflammatory Effect of Turbo cornutus Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study.
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DOI：
10.3390/foods11030364
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发表时间：
2022-01-27
期刊：
影响因子：
--
通讯作者：
Heo SJ
中科院分区：
文献类型：
--
作者：
Kim EA;Kang N;Kim J;Yang HW;Ahn G;Heo SJ
角螺是一种角海螺，分布在潮间带和玄武岩海岸线上，是济州岛重要的渔业资源。本研究以斑马鱼胚胎为实验材料，初步研究了角毛虫70%乙醇提取物在体外对内毒素刺激的RAW264.7细胞的抗炎作用。TVE可减少内毒素刺激的一氧化氮(NO)和前列腺素E_2(PGE_2)的产生，且无任何毒性作用。还可降低脂多糖诱导的诱导型一氧化氮合酶和环氧合酶-2的蛋白表达，抑制肿瘤坏死因子-α、白介素6和白介素1β等促炎细胞因子的产生。此外，机制研究表明，TVE抑制c-jun氨基末端激酶的磷酸化和核因子-kB的激活。在斑马鱼胚胎中，根据存活率和细胞死亡的研究结果，TVE没有表现出发育毒性。在内毒素刺激的斑马鱼胚胎中，TVE抑制NO的产生和细胞死亡。综上所述，初步研究结果表明TVE具有潜在的抗炎作用，可以作为一种功能性食品成分加以开发。
Turbo cornutus, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from T. cornutus viscera (TVE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and zebrafish embryos in vivo. TVE reduced the production of LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) without any toxic effects. TVE also decreased the protein expression of LPS-induced inducible NO synthase and cyclooxygenase-2 and suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, and IL-1β. Furthermore, mechanistic studies indicated that TVE suppressed c-Jun N-terminal kinase phosphorylation and nuclear factor-kB activation. In zebrafish embryos, TVE did not show developmental toxicity based on the survival rate and cell death findings. In LPS-stimulated zebrafish embryos, TVE suppressed NO production and cell death. In conclusion, the result from this preliminary study showed TVE has a potential anti-inflammatory property that can be exploited as a functional food ingredient.
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DOI：
10.3390/plants9101345
发表时间：
2020-10-12
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Chahardehi AM;Arsad H;Lim V
通讯作者：
Lim V
影响因子：
5.4
作者：
Jayawardena TU;Sanjeewa KKA;Nagahawatta DP;Lee HG;Lu YA;Vaas APJP;Abeytunga DTU;Nanayakkara CM;Lee DS;Jeon YJ
通讯作者：
Jeon YJ
影响因子：
2.9
作者：
Mathias, Jonathan R.;Dodd, M. Ernest;Walters, Kevin B.;Yoo, Sa Kan;Ranheim, Erik A.;Huttenlocher, Anna
通讯作者：
Huttenlocher, Anna
影响因子：
5
作者：
Li, Liang;Sapkota, Mahesh;Soh, Yunjo
通讯作者：
Soh, Yunjo
影响因子：
5.6
作者：
Kim, Eun-A;Kim, Seo-Young;Heo, Soo-Jin
通讯作者：
Heo, Soo-Jin