Anti-Inflammatory Effects of Sulfated Polysaccharide from Sargassum Swartzii in Macrophages via Blocking TLR/NF-Κb Signal Transduction.
Anti-Inflammatory Effects of Sulfated Polysaccharide from Sargassum Swartzii in Macrophages via Blocking TLR/NF-Κb Signal Transduction.
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羊栖菜硫酸多糖通过阻断TLR/NF-Κb信号转导对巨噬细胞的抗炎作用
DOI：
10.3390/md18120601
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发表时间：
2020-11-28
期刊：
影响因子：
5.4
通讯作者：
Jeon YJ
中科院分区：
文献类型：
--
作者：
Jayawardena TU;Sanjeewa KKA;Nagahawatta DP;Lee HG;Lu YA;Vaas APJP;Abeytunga DTU;Nanayakkara CM;Lee DS;Jeon YJ
本研究采用酶法提取并经离子交换层析纯化了马尾藻褐藻糖胶。评价了分离的岩藻依聚糖的化学和分子特征，涉及其在LPS诱导条件下在RAW 264.7巨噬细胞中的抗炎潜力。岩藻依聚糖硫酸酯的结构特性通过FTIR和NMR光谱进行了评估。褐藻糖胶可显著抑制LPS刺激的NO生成。这被证明是通过岩藻依聚糖作用于介质如iNOS和考克斯-2，包括促炎细胞因子（TNF-α、IL-6和IL-1β）实现的，具有剂量依赖性下调。此外，该作用通过抑制TLR介导的MyD 88、IKK复合物而表现，最终阻碍NF-κB和MAPK活化，提出其在炎症相关疾病中的治疗应用。研究结果为褐藻岩藻聚糖硫酸酯的可持续利用提供了参考。Swartzii在营养、制药和药妆领域中作为有效的抗炎剂。
This study involves enzymatic extraction of fucoidan from Sargassum swartzii and further purification via ion-exchange chromatography. The chemical and molecular characteristics of isolated fucoidan is evaluated concerning its anti-inflammatory potential in RAW 264.7 macrophages under LPS induced conditions. Structural properties of fucoidan were assessed via FTIR and NMR spectroscopy. NO production stimulated by LPS was significantly declined by fucoidan. This was witnessed to be achieved via fucoidan acting on mediators such as iNOS and COX-2 including pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), with dose dependent down-regulation. Further, the effect is exhibited by the suppression of TLR mediated MyD88, IKK complex, ultimately hindering NF-κB and MAPK activation, proposing its therapeutic applications in inflammation related disorders. The research findings provide an insight in relation to the sustainable utilization of fucoidan from marine brown algae S. swartzii as a potent anti-inflammatory agent in the nutritional, pharmaceutical, and cosmeceutical sectors.
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影响因子：
3.6
作者：
Hwang, Pai-An;Hung, Yu-Lan;Chien, Shih-Yung
通讯作者：
Chien, Shih-Yung
影响因子：
3.5
作者：
Ansari, Abid Ali;Ghanem, Sulaiman Mohammad
通讯作者：
Ghanem, Sulaiman Mohammad
影响因子：
3.1
作者：
Bilan, MI;Grachev, AA;Usov, AI
通讯作者：
Usov, AI
影响因子：
5
作者：
IALENTI, A;IANARO, A;DIROSA, M
通讯作者：
DIROSA, M
岩藻依氨基生物活性的重要决定因素：对棕色海藻中含有岩藻糖硫化的多糖的结构功能关系和提取方法的批判性回顾。
影响因子：
5.4
作者：
Ale MT;Mikkelsen JD;Meyer AS
通讯作者：
Meyer AS