Fluorescence lifetime measurements and biological imaging.
Fluorescence lifetime measurements and biological imaging.
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DOI：
10.1021/cr900343z
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发表时间：
2010-05-12
期刊：
影响因子：
62.1
通讯作者：
Achilefu, Samuel
中科院分区：
文献类型：
--
作者：
Berezin, Mikhail Y.;Achilefu, Samuel
当分子吸收适当能量的光子时，就会发生一系列光物理事件，例如内部转换或振动弛豫（没有光发射时能量损失）、荧光、系间窜越（从单重态到三重态）和磷光，如有机分子的 Jablonski 图所示（图 1）。每个过程都以一定的概率发生，以衰减率常数 (k) 为特征。可以证明，一组分子从一种状态衰变到另一种状态的平均时间长度 τ 与衰变速率成反比：τ) 1/k。这个平均时间长度称为平均寿命或简称为寿命。还可以证明，光物理过程的寿命是N个电子激发分子群体减少e倍所需的时间。相应地，荧光寿命是一群受激发的荧光团通过荧光和其他非辐射过程的能量损失而呈指数减少到 N/e 所需的时间。光物理的寿命
When a molecule absorbs a photon of appropriate energy, a chain of photophysical events ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence of light emission), fluorescence, intersystem crossing (from singlet state to a triplet state), and phosphorescence, as shown in the Jablonski diagram for organic molecules (Figure 1). Each of the processes occurs with a certain probability, characterized by decay rate constants (k). It can be shown that the average length of time τ for the set of molecules to decay from one state to another is reciprocally proportional to the rate of decay: τ) 1/k. This average length of time is called the mean lifetime or simply lifetime. It can also be shown that the lifetime of a photophysical process is the time required by a population of N electronically excited molecules to be reduced by a factor of e. Correspondingly, the fluorescence lifetime is the time required by a population of excited fluorophores to decrease exponentially to N/e via the loss of energy through fluorescence and other nonradiative processes. The lifetime of photophycal
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影响因子：
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作者：
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DOI：
10.1073/pnas.0706806104
发表时间：
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影响因子：
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作者：
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