Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce (var. ramosa Hort.)
Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce (var. ramosa Hort.)
复制标题
嗜温冷杆菌 sp.
DOI：
10.1007/s10482-019-01287-3
复制
发表时间：
2019-06
期刊：
影响因子：
--
通讯作者：
Wang Xiangjing
中科院分区：
文献类型：
--
作者：
Wang Ying;Cao Peng;Sun Peng;Zhao Junwei;Sun Xiujun;Zhang Ji;Li Chenxu;Xiang Wensheng;Wang Xiangjing
从莴苣根际土壤中分离到一株革兰氏染色阳性、需氧、杆状细菌，命名为NEAU-85 T，并使用多相方法表征。发现菌株NEAU-85 T是过氧化氢酶阳性的，能动的并且能够在10 ° C和30 °C之间生长。基于16 S rRNA基因序列的系统发育分析表明，菌株NEAU-85 T属于Cryobacterium属，与模式菌株Cryobacterium psychrotolerans JCM 13925 T（98.5%）和Leifsonia kafniensis NCCB 100216 T（97.6%）的亲缘关系最近。利用atpD、dnaK、recA、rpoB、sevY和ychF基因的串联基因序列进行多位点序列分析，结果表明菌株NEAU-85 T属于低温杆菌属。DNA数字杂交结果表明，NEAU-85 T与其亲缘关系最近的C.耐心理动物甲基萘醌被鉴定为MK-10和MK-11。发现磷脂谱由二磷脂酰甘油、磷脂酰甘油、磷脂、未鉴别糖脂和未鉴别脂质组成。主要脂肪酸为anteiso-C15：0、anteiso-C15：1和iso-C16：0。测定了菌株NEAU-85 T基因组DNA的G + C含量为68.9mol%。它们之间的DNA-DNA杂交值小于70%。根据表型、基因型和系统发育数据，菌株NEAU-85 T可被认为是低温杆菌属的一个新种，并建议将其命名为嗜暗低温杆菌（Cryobacterium tepidiphilum sp. nov.）。模式菌株为NEAU-85 T（= CCTCC AA 2018035 T = JCM 32545 T）。
A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85T, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85Twas found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85Tbelongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925T(98.5%) and also to Leifsonia kafniensis NCCB 100216T(97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85Tbelongs to the genus Cryobacterium. The digital DNA-DNA hybridization values revealed that strain NEAU-85Tis distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C15:0, anteiso-C15:1and iso-C16:0. The genomic DNA G + C content of strain NEAU-85Twas determined to be 68.9 mol%. The DNA-DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85Tcan be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85T(= CCTCC AA 2018035T= JCM 32545T).
登录
查看更多内容
DOI：
10.1099/ijs.0.64750-0
发表时间：
2007-04
影响因子：
2.8
作者：
Dechao Zhang;He-Xiang Wang;Heng-Lin Cui;Yong Yang;Hong-Can Liu;Xiuzhu Dong;P. Zhou
通讯作者：
Dechao Zhang;He-Xiang Wang;Heng-Lin Cui;Yong Yang;Hong-Can Liu;Xiuzhu Dong;P. Zhou
影响因子：
1.2
作者：
Kôzô Inoue
通讯作者：
Kôzô Inoue
DOI：
--
发表时间：
1961
期刊：
--
影响因子：
--
作者：
S. Waksman
通讯作者：
S. Waksman
DOI：
10.1099/00207713-47-2-474
发表时间：
1997-04
期刊：
International journal of systematic bacteriology
影响因子：
--
作者：
K. Suzuki;J. Sasaki;M. Uramoto;T. Nakase;K. Komagata
通讯作者：
K. Suzuki;J. Sasaki;M. Uramoto;T. Nakase;K. Komagata
DOI：
10.1007/s10482-017-0841-7
发表时间：
2017-02
期刊：
Antonie van Leeuwenhoek
影响因子：
--
作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe
通讯作者：
R. Rosselló-Móra;M. Trujillo;I. Sutcliffe