Correlative two-color two-photon (2C2P) excitation STED microscopy.
Correlative two-color two-photon (2C2P) excitation STED microscopy.
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相关双色双光子 (2C2P) 激发 STED 显微镜。
DOI：
10.1364/boe.10.004516
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发表时间：
2019
影响因子：
3.4
通讯作者：
T. Hellerer
中科院分区：
文献类型：
--
作者：
C. Polzer;Stefan Ness;M. Mohseni;T. Kellerer;M. Hilleringmann;J. Rädler;T. Hellerer
我们提出了一种双色双光子受激发射耗尽显微镜技术(2C2P-STED)，它利用非线性激发的固有自参考机制将共焦图像与超分辨图像相关联。这种新的方法克服了激光扫描荧光显微镜中两种不同成像模式在纳米尺度上进行共定位所带来的巨大挑战。演示了2C2P-STED的原理，我们首次展示了革兰氏阳性细菌肺炎链球菌TIGR4型菌毛的超分辨率图像。在2C2P激发模式下，信噪比大于10，在2P-STED中可分辨约70 nm细节。
We present a two-color two-photon stimulated emission depletion microscopy technique (2C2P-STED) that correlates a confocal image with a super-resolved image employing the inherent self-referencing mechanism of nonlinear excitation. The novel approach overcomes the substantial challenge posed by two different imaging modalities in laser-scanning fluorescence microscopy for colocalization on the nanometer scale. Demonstrating the principle of 2C2P-STED, we show for the first time super-resolved images of the gram-positive bacteria Streptococcus pneumoniae TIGR4 pilus type-1. A signal-to-noise ratio (SNR) greater than 10 was achieved in 2C2P excitation mode and approximately 70 nm details were resolved in 2P-STED.
影响因子：
6.8
作者：
Caplan J;Niethammer M;Taylor RM 2nd;Czymmek KJ
通讯作者：
Czymmek KJ
影响因子：
56.9
作者：
DENK, W;STRICKLER, JH;WEBB, WW
通讯作者：
WEBB, WW