Thapsigargin, a Ca2+‐ATPase inhibitor, depletes the intracellular Ca2+ pool and induces apoptosis in human hepatoma cells.
Thapsigargin, a Ca2+‐ATPase inhibitor, depletes the intracellular Ca2+ pool and induces apoptosis in human hepatoma cells.
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Thapsigargin 是一种 Ca2+-ATPase 抑制剂，可消耗细胞内 Ca2+ 池并诱导人肝癌细胞凋亡。
DOI：
10.1006/cbir.1993.1021
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发表时间：
1993
影响因子：
3.9
通讯作者：
Y. Kaneko
中科院分区：
文献类型：
--
作者：
A. Tsukamoto;Y. Kaneko
图 1. 毒胡萝卜素对细胞生长的影响 (A)、DNA 片段化 (B) 以及 EGF 对 [Ca'"]! 的作用 (C)。0 -，对照； • Tg，毒胡萝卜素；M，<j>X174 DNNHae III 消化，在连续存在毒胡萝卜素 (:2:5 ng/ml) 的情况下培养时，PLC/PRF/5 肝癌细胞的增殖受到抑制。此外，用 30 ng/ml 毒胡萝卜素处理 3 小时，然后在不含毒胡萝卜素的培养基中培养的细胞的 DNA 片段化也受到抑制（图 A）。在这两种情况下，培养 4 天后，细胞变小，细胞质变窄，细胞核破碎，尽管这些细胞中 98% 以上仍能够排除台盼蓝染料，但在这个阶段，DNA 片段已可识别（图 B）。毒胡萝卜素将对照细胞的 [Ca2+]i 从 118 nM 升高至 461 nM，EGF（肝癌细胞生长的刺激剂）将对照细胞的 [Ca2+]i 升高至 435 nM，但是，EGF 不会增加用 5 ng/rnl 毒胡萝卜素预培养 3 天的细胞的 [Ca2+]i（图 C）。
Figure 1. Effects of thapsigargin on the cell growth (A), DNA fragmentation (B), and action of EGF on [Ca'"]! (C). 0 -, control; • Tg, thapsigargin; M, <j>X174 DNNHae III digest, The proliferation of PLC/PRF/5 hepatoma cells was suppressed on culturing in the continuous presence of thapsigargin (:2:5 ng/ml). In addition, that of the cells treated for 3 h with 30 ng/ml of thapsigargin and then cultured in thapsigargin-free medium was also inhibited (Fig.A) . In either case, the cells became small with narrow cytoplasm and fragmented nuclei after 4 days of culture. Although more than 98% of these cells were still capable of excluding trypan blue dye, DNA fragmentation was recognizable already at this stage (Fig. B). Then, the cells gradually detached from the culture dish and were dead. Thapsigargin elevated [Ca of control cells from 118 to 461 nM. EGF, a stimulator of hepatoma cell growth, elevated [Ca2+]i of control cells to 435 nM, However, EGF did not increase [Ca2+]i of cells precultured for 3 days with 5 ng/rnl ofthapsigargin (Fig. C),
影响因子：
4.8
作者：
KYPRIANOU, N;ISAACS, JT
通讯作者：
ISAACS, JT