Functional Insights into Recombinant TROSPA Protein from Ixodes ricinus
Functional Insights into Recombinant TROSPA Protein from Ixodes ricinus
复制标题
蓖麻硬蜱重组 TROSPA 蛋白的功能研究
作者：
M. Figlerowicz;A. Urbanowicz;Dominik Lewandowski;J. Jodynis;C. Sadowski
莱姆病（也称为疏螺旋体病）是一种由蜱传播并由伯氏疏螺旋体引起的流行性慢性疾病。L.螺旋体至少一种蜱蛋白，即来自I.通常发生在美国的肩胛肌是细菌定殖载体所必需的。TROSPA位于蜱肠道中，与螺旋体外表面蛋白A（OspA）相互作用并启动蜱定殖。蓖麻硬蜱是参与B的主要载体。布格多费里湾L.在大多数欧洲国家传播。在本研究中，我们表征了来自I.在一些实施方案中，本发明的组合物用于与来自不同疏螺旋体属物种的OspA相互作用并在动物中诱导免疫应答。我们还表明，TROSPA的N-末端部分（推定的跨膜结构域）不参与与OspA的相互作用，并且TROSPA蛋白上的总负电荷的减少损害了TROSPA-OspA结合。一般而言，本文提供的数据表明，重组TROSPA蛋白保留了与OspA形成复合物的能力，并在口服免疫的大鼠中诱导显著水平的IgG。因此，我。蓖麻TROSPA可以被认为是抗疏螺旋体的动物疫苗的良好候选组分。
Lyme disease (also called borreliosis) is a prevalent chronic disease transmitted by ticks and caused by Borrelia burgdorferi s. l. spirochete. At least one tick protein, namely TROSPA from I. scapularis, commonly occurring in the USA, was shown to be required for colonization of the vector by bacteria. Located in the tick gut, TROSPA interacts with the spirochete outer surface protein A (OspA) and initiates the tick colonization. Ixodes ricinus is a primary vector involved in B. burgdorferi s. l. transmission in most European countries. In this study, we characterized the capacities of recombinant TROSPA protein from I. ricinus to interact with OspA from different Borrelia species and to induce an immune response in animals. We also showed that the N-terminal part of TROSPA (a putative transmembrane domain) is not involved in the interaction with OspA and that reduction of the total negative charge on the TROSPA protein impaired TROSPA-OspA binding. In general, the data presented in this paper indicate that recombinant TROSPA protein retains the capacity to form a complex with OspA and induces a significant level of IgG in orally immunized rats. Thus, I. ricinus TROSPA may be considered a good candidate component for an animal vaccine against Borrelia.
影响因子：
3.1
作者：
Kung F;Anguita J;Pal U
通讯作者：
Pal U